ArticleArchives of microbiology2016
A set of isogenic auxotrophic strains for constructing multiple gene deletion mutants and parasexual crossings in Aspergillus niger.
Article in Archives of microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 29 citations in OpenAlex.
- Constructing pyrG marker by CRISPR/Cas facilities the highly-efficient precise genome editing on industrial Aspergillus niger strain.Bioprocess and biosystems engineering · 2025Article
- Engineering of Aspergillus niger for efficient production of D-xylitol from L-arabinose.Microbial cell factories · 2024Article
- A novel luciferase-based reporter tool to monitor the dynamics of carbon catabolite repression in filamentous fungi.Microbial biotechnology · 2024Article
- Genetically Engineered Microorganisms and Their Impact on Human Health.International journal of clinical practice · 2024Review
- Development of a new Agrobacterium-mediated transformation system based on a dual auxotrophic approach in the filamentous fungus Aspergillus oryzae.World journal of microbiology & biotechnology · 2021Article
- Genetic Manipulation and Transformation Methods forMycobiology · 2021Review
- Genetic Characterization of Mutations Related to Conidiophore Stalk Length Development inFrontiers in genetics · 2021Article
- Interrogation of the cell wall integrity pathway inGene: X · 2020Article
- Comparative evaluation of Aspergillus niger strains for endogenous pectin-depolymerization capacity and suitability for D-galacturonic acid production.Bioprocess and biosystems engineering · 2020Article
- Regulating Strategies for Producing Carbohydrate Active Enzymes by Filamentous Fungal Cell Factories.Frontiers in bioengineering and biotechnology · 2020Review
- Mutations in AraR leading to constitutive expression of arabinolytic genes in Aspergillus niger under derepressing conditions [corrected].Applied microbiology and biotechnology · 2019Article
- Efficient marker free CRISPR/Cas9 genome editing for functional analysis of gene families in filamentous fungi.Fungal biology and biotechnology · 2019Article
- Polycistronic gene expression in Aspergillus niger.Microbial cell factories · 2017Article
- HisB as novel selection marker for gene targeting approaches in Aspergillus niger.BMC microbiology · 2017Article
- Diverse data supports the transition of filamentous fungal model organisms into the post-genomics era.Mycology · 2017Article
- Article
- Transcriptomic and molecular genetic analysis of the cell wall salvage response of Aspergillus niger to the absence of galactofuranose synthesis.Cellular microbiology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To construct a set of isogenic auxotrophic strains in Aspergillus niger suited for creating multiple gene deletion mutants and executing parasexual crossings, we have combined mutations in genes involved in colour pigmentation (fwnA and olvA) with well-selectable auxotrophic markers (pyrG, nicB, argB, and adeA). All markers, except for the pyrG marker, were introduced by targeted deletion, omitting UV mutagenesis of the strains. Aspergillus oryzae orthologous genes of the argB, nicB, and adeA markers were used as heterologous selection markers, and all markers were shown to complement to respective auxotrophic A. niger mutants. A quadruple auxotrophic marker was further constructed suitable for multiple gene deletions. Genome sequencing of two auxotrophic colour mutants JN3.2 (olvA::pyrG, argB::hygB) and JN6.2 (olvA::pyrG, nicB::hygB) revealed four SNPs between them in non-coding regions, indicating a high level of isogenicity between both strains. The availability of near-isogenic complementary auxotrophic colour mutants facilitates the selection of diploids and the isolation of haploid segregants from the diploid using the parasexual cycle.
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