ArticleScientific reports2016
Molecular mechanism and functional significance of acid generation in the Drosophila midgut.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
58 citing papers in PubMed.
- RNAseK is vital for epithelial function in Drosophila melanogaster.The Journal of experimental biology · 2026Article
- Context-dependent activation and evolutionary buffering of a mating pheromone in fission yeast.Communications biology · 2026Article
- Caenorhabditis elegans as an experimental model for resilience-boosting microbiota interventions in nonmodel ectotherms.FEMS microbiology reviews · 2026Review
- Selected gut bacteria promote mosquito larval growth through the regulation of ionic microenvironment and protease expression.iScience · 2025Article
- Alkaline-Acid Intestine Environment Controlled by A Carbonic Anhydrase Gene Influences Synthesis of Sex Pheromone by Symbionts.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Gut-brain communication in Drosophila melanogaster.Current opinion in neurobiology · 2025Review
- New molecular components of high and low affinity iron import systems in Drosophila.Nature communications · 2025Article
- Microbial Community Dynamics in Natural Drosophila melanogaster Populations Across Seasons.Environmental microbiology · 2025Article
- Evolution of resistance and disease tolerance mechanisms to oral bacterial infection inOpen biology · 2025Article
- The integral role of the Black Soldier fly,Frontiers in microbiomes · 2025Review
- Microbiome Associated with Polypedilum sp. (Diptera; Chironomidae), a Midge Adapted to an Extremely Acidic Environment.Microbes and environments · 2025Article
- Notch-Dependent Expression of theGenes · 2024Article
- Microbiota-derived acetylcholine can promote gut motility inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Article
- The role of animal hosts in shaping gut microbiome variation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Factors Related toLife (Basel, Switzerland) · 2024Article
- Structural and Functional Characterization ofMolecules (Basel, Switzerland) · 2023Article
- Intestinal GCN2 controlseLife · 2023Article
- Post-feeding transcriptomics reveals essential genes expressed in the midgut of the desert locust.Frontiers in physiology · 2023Article
- Powerful, efficient QTL mapping in Drosophila melanogaster using bulked phenotyping and pooled sequencing.Genetics · 2022Article
- Nutrient Sensing via Gut inInternational journal of molecular sciences · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
The gut of Drosophila melanogaster includes a proximal acidic region (~pH 2), however the genome lacks the H(+)/K(+) ATPase characteristic of the mammalian gastric parietal cell, and the molecular mechanisms of acid generation are poorly understood. Here, we show that maintenance of the low pH of the acidic region is dependent on H(+) V-ATPase, together with carbonic anhydrase and five further transporters or channels that mediate K(+), Cl(-) and HCO3(-) transport. Abrogation of the low pH did not influence larval survival under standard laboratory conditions, but was deleterious for insects subjected to high Na(+) or K(+) load. Insects with elevated pH in the acidic region displayed increased susceptibility to Pseudomonas pathogens and increased abundance of key members of the gut microbiota (Acetobacter and Lactobacillus), suggesting that the acidic region has bacteriostatic or bacteriocidal activity. Conversely, the pH of the acidic region was significantly reduced in germ-free Drosophila, indicative of a role of the gut bacteria in shaping the pH conditions of the gut. These results demonstrate that the acidic gut region protects the insect and gut microbiome from pathological disruption, and shed light on the mechanisms by which low pH can be maintained in the absence of H(+), K(+) ATPase.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.