Observational studyJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2016
Comparison of Two ELISA Methods and Mass Spectrometry for Measurement of Vitamin D-Binding Protein: Implications for the Assessment of Bioavailable Vitamin D Concentrations Across Genotypes.
Observational study in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 107 citations in OpenAlex.
- Serum Vitamin D: Correlates of Baseline Concentration and Response to Supplementation in VITAL-DKD.The Journal of clinical endocrinology and metabolism · 2022Trial
- Randomized phase 2 trial of monthly vitamin D to prevent respiratory complications in children with sickle cell disease.Blood advances · 2018Trial
- Racial differences in calculated bioavailable vitamin D with vitamin D/calcium supplementation.AIDS (London, England) · 2017Trial
- Impact of high-dose vitamin DNutrition (Burbank, Los Angeles County, Calif.) · 2017Trial
- Effects of Cholecalciferol vs Calcifediol on Total and Free 25-Hydroxyvitamin D and Parathyroid Hormone.The Journal of clinical endocrinology and metabolism · 2017Trial
- Effects of cholecalciferol supplementation on serum and urinary vitamin D metabolites and binding protein in HIV-infected youth.The Journal of steroid biochemistry and molecular biology · 2017Trial
- Early pregnancy vitamin D status and risk of preeclampsia.The Journal of clinical investigation · 2016Trial
- Advances in the measurement of vitamin D binding protein.Analytical and bioanalytical chemistry · 2026Review
- Vitamin D: A Bridge between Kidney and Heart.Life (Basel, Switzerland) · 2024Review
- Vitamin D Binding Protein and Postsurgical Outcomes and Tissue Injury Markers After Hip Fracture: A Prospective Study.The Journal of clinical endocrinology and metabolism · 2023Article
- Inaccuracies in plasma oxytocin extraction and enzyme immunoassay techniques.Comprehensive psychoneuroendocrinology · 2023Article
- Genetic correlates of vitamin D-binding protein and 25-hydroxyvitamin D in neonatal dried blood spots.Nature communications · 2023Article
- Vitamin D Metabolites: Analytical Challenges and Clinical Relevance.Calcified tissue international · 2023Review
- Plasma sterols and vitamin D are correlates and predictors of ozone-induced inflammation in the lung: A pilot study.PloS one · 2023Article
- Review
- Serum 25-Hydroxyvitamin D is Associated With Bone Microarchitecture and Strength in a Multiracial Cohort of Young Adults.The Journal of clinical endocrinology and metabolism · 2022Article
- Haplotypes in theNutrients · 2022Article
- Article
- Recommendations on the measurement and the clinical use of vitamin D metabolites and vitamin D binding protein - A position paper from the IFCC Committee on bone metabolism.Clinica chimica acta; international journal of clinical chemistry · 2021Review
- Fibrinogen and hemoglobin predict near future cardiovascular events in asymptomatic individuals.Scientific reports · 2021Article
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11 authors at 6 institutions in 1 country.
Funding
Abstract
Studies using vitamin D-binding protein (DBP) concentrations to estimate free and bioavailable vitamin D have increased dramatically in recent years. Combinations of two single-nucleotide polymorphisms (SNPs) produce three major DBP isoforms (Gc1f, Gc1s, and Gc2). A recent study showed that DBP concentrations quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS) did not differ by race, whereas a widely used monoclonal enzyme-linked immunosorbent assay (ELISA) quantified DBP differentially by isoform, yielding significantly lower DBP concentrations in black versus white individuals. We compared measurements of serum DBP using a monoclonal ELISA, a polyclonal ELISA, and LC-MS/MS in 125 participants in the Chronic Renal Insufficiency Cohort (CRIC). Serum free and bioavailable 25OHD were calculated based on DBP concentrations from these three assays in homozygous participants, and race differences were compared. We confirmed that the monoclonal ELISA quantifies DBP differentially by isoform and showed that the polyclonal ELISA is not subject to this bias. Whereas ≤9% of the variability in DBP concentrations quantified using either LC-MS/MS or the polyclonal ELISA was explained by genotype, 85% of the variability in the monoclonal ELISA-based measures was explained by genotype. DBP concentrations measured by the monoclonal ELISA were disproportionately lower than LC-MS/MS-based results for Gc1f homozygotes (median difference -67%; interquartile range [IQR] -71%, -64%), 95% of whom were black. In contrast, the polyclonal ELISA yielded consistently and similarly higher measurements of DBP than LC-MS/MS, irrespective of genotype, with a median percent difference of +50% (IQR +33%, +65%). Contrary to findings using the monoclonal ELISA, DBP concentrations did not differ by race, and free and bioavailable 25OHD were significantly lower in black versus white participants based on both the polyclonal ELISA and LC-MS/MS, consistent with their lower total 25OHD. Future studies of DBP and free or bioavailable vitamin D metabolites should employ DBP assays that are not biased by DBP genotype. © 2016 American Society for Bone and Mineral Research.
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