ArticleBMC musculoskeletal disorders2016
The osteoblast as an inflammatory cell: production of cytokines in response to bacteria and components of bacterial biofilms.
Article in BMC musculoskeletal disorders, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 50 citations in OpenAlex.
- The Innate and Adaptive Immune Functions of Osteoblast-Lineage Cells.Current osteoporosis reports · 2026Review
- Establishment of a three-dimensional in vitro peri-implant bone-mucosa composite model.BMC oral health · 2026Article
- Deep-freezing and decontamination strategy for a large autologous bone graft with presentation of the osteogenic potential of resident osteoblasts.Stem cell research & therapy · 2025Article
- Interleukin 26 attenuates osteoblast differentiation in osteoarthritis patients by activating COX2 and NF-κB pathways.International journal of medical sciences · 2025Article
- Non-biofilm-forming Staphylococcus epidermidis planktonic cell supernatant induces alterations in osteoblast biological function.Scientific reports · 2024Article
- Secretome analysis and virulence assessment inJournal of oral microbiology · 2024Article
- Article
- Inflammatory Processes Affecting Bone Health and Repair.Current osteoporosis reports · 2023Review
- Comparative Study of the Cytokine Profiles of Serum and Tissues from Patients with the Ossification of the Posterior Longitudinal Ligament.Biomedicines · 2023Article
- Review
- A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials.Journal of visualized experiments : JoVE · 2023Article
- Profiling the Immune Response to Periprosthetic Joint Infection and Non-Infectious Arthroplasty Failure.Antibiotics (Basel, Switzerland) · 2023Review
- Dual-functional composite scaffolds for inhibiting infection and promoting bone regeneration.Materials today. Bio · 2022Review
- Human Osteoblast-Conditioned Media Can InfluenceInternational journal of molecular sciences · 2022Article
- Cellular Response of Human Osteoblasts to Different Presentations of Deproteinized Bovine Bone.Materials (Basel, Switzerland) · 2022Article
- The osteoblast secretome inFrontiers in immunology · 2022Review
- Fracture biomechanics influence local and systemic immune responses in a murine fracture-related infection model.Biology open · 2021Article
- Patient-specific effects of soluble factors from Staphylococcus aureus and Staphylococcus epidermidis biofilms on osteogenic differentiation of primary human osteoblasts.Scientific reports · 2021Article
- Anacardic acid-mediated regulation of osteoblast differentiation involves mitigation of inflammasome activation pathways.Molecular and cellular biochemistry · 2021Article
- Acid-Induced Inflammatory Cytokines in Osteoblasts: A Guided Path to Osteolysis in Bone Metastasis.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundImplant infections are a major complication in the field of orthopaedics. Bacteria attach to the implant-surface and form biofilm-colonies which makes them difficult to treat. Not only immune cells exclusively respond to bacterial challenges, but also local tissue cells are capable of participating in defense mechanisms. The aim of this study was to evaluate the role of osteoblasts in the context of implant infections.
methodsPrimary osteoblasts were cultivated and stimulated with free-swimming bacteria at 4 °C and 37 °C. Supernatants were harvested for ELISA and expression of pro-inflammatory cytokines evaluated by RT-PCR. Bacterial binding to osteoblasts was evaluated using cytofluorometry and uptake was investigated by (3)H thymidine-labelling of bacteria. Osteoblasts were additionally stimulated with the extracellular polymeric substance (EPS) of Staphylococcus epidermidis biofilms, as well as components of the EPS; the bacterial heat shock protein GroEL in particular.
resultsWe demonstrated that binding of bacteria to the osteoblast cell surface leads to an increased production of pro-inflammatory cytokines. Bacteria are capable of surviving intracellular. Furthermore, osteoblasts do not only respond to free-swimming, planktonic bacteria, but also to components of the EPS, including lipoteichoic acid and the heat shock protein GroEL.
conclusionIn conclusion, local tissue cells, specifically osteoblasts, might contribute to the persistence of the inflammatory response associated with implant-infections.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.