Evidence map›Paper›PMID 27249377›Full record

ArticleElectrophoresis2016

Deciphering glycomics and neuroproteomic alterations in experimental traumatic brain injury: Comparative analysis of aspirin and clopidogrel treatment.

Hussein Abou-Abbass, Hisham Bahmad, Hadi Abou-El-Hassan, Rui Zhu, Shiyue Zhou, Xue Dong, Eva Hamade, Khalil Mallah, Abir Zebian, Naify Ramadan and 8 more

Open access · greenAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Electrophoresis, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
1.6field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
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  11. The dynamic brain N-glycome.Glycoconjugate journal · 2022
    Review
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  13. Article
  14. Article
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  17. 8-plex LC-MS/MS Analysis of PermethylatedAnalytical chemistry · 2019
    Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 7 institutions in 3 countries.

Hussein Abou-AbbassDepartment of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.
Hisham BahmadFaculty of Medicine, Beirut Arab University, Beirut, Lebanon.
Hadi Abou-El-HassanFaculty of Medicine, American University of Beirut, Beirut, Lebanon.
Rui ZhuDepartment of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Shiyue ZhouDepartment of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Xue DongDepartment of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Eva HamadeER045-Laboratory of Stem Cells, DSST, Lebanese University, Beirut, Lebanon.
Khalil MallahER045-Laboratory of Stem Cells, DSST, Lebanese University, Beirut, Lebanon.
Abir ZebianDepartment of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.
Naify RamadanDepartment of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.
Stefania MondelloDepartment of Neurosciences, University of Messina, Messina, Italy.
Jawad FaresFaculty of Medicine, American University of Beirut, Beirut, Lebanon.
Youssef ComairDepartment of Surgery, Division of Neurosurgery, Lebanese American University, Beirut, Lebanon.
Samir AtwehDepartment of Neurology, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.
Hala DarwishFaculty of Medicine-School of Nursing, American University of Beirut, New York, NY, USA.
Kazem ZibaraER045-Laboratory of Stem Cells, DSST, Lebanese University, Beirut, Lebanon.
Yehia MechrefDepartment of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Firas KobeissyDepartment of Biochemistry and Molecular Genetics, Faculty of Medicine, American University of Beirut, Beirut, Lebanon.
American University of Beirut · LBTexas Tech University · USLebanese University · LBAmerican University of Beirut New York Office · USBeirut Arab University · LBLebanese American University · LBUniversity of Messina · IT

Funding

Sensitive and Quantitative MS-bases Glycomic Mapping PlatformR01GM112490 · NIGMS · TEXAS TECH UNIVERSITY · PI MECHREF, YEHIA · 2014 to 2024
$3.2M
NIGMS NIH HHS R01 GM112490
6 · The paper itself

Abstract

As populations age, the number of patients sustaining traumatic brain injury (TBI) and concomitantly receiving preinjury antiplatelet therapy such as aspirin (ASA) and clopidogrel (CLOP) is rising. These drugs have been linked with unfavorable clinical outcomes following TBI, where the exact mechanism(s) involved are still unknown. In this novel work, we aimed to identify and compare the altered proteome profile imposed by ASA and CLOP when administered alone or in combination, prior to experimental TBI. Furthermore, we assessed differential glycosylation PTM patterns following experimental controlled cortical impact model of TBI, ASA, CLOP, and ASA + CLOP. Ipsilateral cortical brain tissues were harvested 48 h postinjury and were analyzed using an advanced neuroproteomics LC-MS/MS platform to assess proteomic and glycoproteins alterations. Of interest, differential proteins pertaining to each group (22 in TBI, 41 in TBI + ASA, 44 in TBI + CLOP, and 34 in TBI + ASA + CLOP) were revealed. Advanced bioinformatics/systems biology and clustering analyses were performed to evaluate biological networks and protein interaction maps illustrating molecular pathways involved in the experimental conditions. Results have indicated that proteins involved in neuroprotective cellular pathways were upregulated in the ASA and CLOP groups when given separately. However, ASA + CLOP administration revealed enrichment in biological pathways relevant to inflammation and proinjury mechanisms. Moreover, results showed differential upregulation of glycoproteins levels in the sialylated N-glycans PTMs that can be implicated in pathological changes. Omics data obtained have provided molecular insights of the underlying mechanisms that can be translated into clinical bedside settings.

Indexed as

AnimalsAspirinBrain Injuries, TraumaticCerebellar CortexClopidogrelGene Expression RegulationGlycomicsGlycosylationHumansMalePlatelet Aggregation InhibitorsProtein Processing, Post-TranslationalProteomicsRatsRats, Sprague-DawleyTiclopidineAspirinClopidogrelPlatelet Aggregation InhibitorsTiclopidineAspirinClopidogrelNeuroproteomicsPTMsTBI

Identifiers

PMID27249377
PMCPMC4963819
OpenAlexW2414560298

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.