ArticleOpen biology2016
ESCRT-II controls retinal axon growth by regulating DCC receptor levels and local protein synthesis.
Article in Open biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- A human DCC variant causing mirror movement disorder reveals that the WAVE regulatory complex mediates axon guidance by netrin-1-DCC.Science signaling · 2024Article
- Bi-allelic variants in SNF8 cause a disease spectrum ranging from severe developmental and epileptic encephalopathy to syndromic optic atrophy.American journal of human genetics · 2024Article
- The functional organization of axonal mRNA transport and translation.Nature reviews. Neuroscience · 2021Review
- Axonal precursor miRNAs hitchhike on endosomes and locally regulate the development of neural circuits.The EMBO journal · 2020Article
- The Role of Dynamic miRISC During Neuronal Development.Frontiers in molecular biosciences · 2020Review
- Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.eLife · 2019Article
- The multi PAM2 protein Upa2 functions as novel core component of endosomal mRNA transport.EMBO reports · 2019Article
- The endosomal sorting adaptor HD-PTP is required for ephrin-B:EphB signalling in cellular collapse and spinal motor axon guidance.Scientific reports · 2019Article
- ESCRT Proteins Control the Dendritic Morphology of Developing and Mature Hippocampal Neurons.Molecular neurobiology · 2019Article
- Article
- ESCRT Mutant Analysis and Imaging of ESCRT Components in the Model Fungus Ustilago maydis.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Local Translation in Axons: When Membraneless RNP Granules Meet Membrane-Bound Organelles.Frontiers in molecular biosciences · 2019Review
- The RNA-binding complex ESCRT-II inThe Journal of biological chemistry · 2018Article
- Cue-Polarized Transport of β-actin mRNA Depends on 3'UTR and Microtubules in Live Growth Cones.Frontiers in cellular neuroscience · 2018Article
- Article
- The ESCRT regulator Did2 maintains the balance between long-distance endosomal transport and endocytic trafficking.PLoS genetics · 2017Article
- Where does axon guidance lead us?F1000Research · 2017Review
- MEX3C interacts with adaptor-related protein complex 2 and involves in miR-451a exosomal sorting.PloS one · 2017Article
- Hitting the mark: Localization of mRNA and biomolecular condensates in health and disease.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Endocytosis and local protein synthesis (LPS) act coordinately to mediate the chemotropic responses of axons, but the link between these two processes is poorly understood. The endosomal sorting complex required for transport (ESCRT) is a key regulator of cargo sorting in the endocytic pathway, and here we have investigated the role of ESCRT-II, a critical ESCRT component, in Xenopus retinal ganglion cell (RGC) axons. We show that ESCRT-II is present in RGC axonal growth cones (GCs) where it co-localizes with endocytic vesicle GTPases and, unexpectedly, with the Netrin-1 receptor, deleted in colorectal cancer (DCC). ESCRT-II knockdown (KD) decreases endocytosis and, strikingly, reduces DCC in GCs and leads to axon growth and guidance defects. ESCRT-II-depleted axons fail to turn in response to a Netrin-1 gradient in vitro and many axons fail to exit the eye in vivo These defects, similar to Netrin-1/DCC loss-of-function phenotypes, can be rescued in whole (in vitro) or in part (in vivo) by expressing DCC. In addition, ESCRT-II KD impairs LPS in GCs and live imaging reveals that ESCRT-II transports mRNAs in axons. Collectively, our results show that the ESCRT-II-mediated endocytic pathway regulates both DCC and LPS in the axonal compartment and suggest that ESCRT-II aids gradient sensing in GCs by coupling endocytosis to LPS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.