ArticleProceedings of the National Academy of Sciences of the United States of America2016
Sphingomyelin is sorted at the trans Golgi network into a distinct class of secretory vesicle.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 120 citations in OpenAlex.
- Sphingolipids and Atherosclerosis.Current atherosclerosis reports · 2026Review
- Leaflet-specific phospholipid imaging using genetically encoded proximity sensors.Nature chemical biology · 2026Article
- Lipid Metabolism and Trafficking in the Golgi Apparatus.Sub-cellular biochemistry · 2026Review
- Article
- Emerging Approaches for Studying Lipid Dynamics, Metabolism, and Interactions in Cells.Annual review of biochemistry · 2025Review
- Magnetic control of membrane damage in early endosomes using internalized magnetic nanoparticles.Cell structure and function · 2025Article
- The danger of flipping an outside lipid to the inside.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Article
- Article
- A non-toxic equinatoxin-II reveals the dynamics and distribution of sphingomyelin in the cytosolic leaflet of the plasma membrane.Scientific reports · 2024Article
- Article
- Calcium flow at ER-TGN contact sites facilitates secretory cargo export.Molecular biology of the cell · 2024Article
- Sorting of secretory proteins at theeLife · 2024Article
- Sorting of complex sphingolipids within the cellular endomembrane systems.Frontiers in cell and developmental biology · 2024Review
- Molecular mechanisms of polarized transport to the apical plasma membrane.Frontiers in cell and developmental biology · 2024Review
- Lipid exchange at ER-trans-Golgi contact sites governs polarized cargo sorting.The Journal of cell biology · 2024Article
- Nanoreporter Identifies Lysosomal Storage Disease Lipid Accumulation Intracranially.Nano letters · 2023Article
- Cholesterol-dependent homeostatic regulation of very long chain sphingolipid synthesis.The Journal of cell biology · 2023Article
- Lipid Sorting and Organelle Identity.Cold Spring Harbor perspectives in biology · 2023Review
- Altered plasma membrane abundance of the sulfatide-binding protein NF155 links glycosphingolipid imbalances to demyelination.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
One of the principal functions of the trans Golgi network (TGN) is the sorting of proteins into distinct vesicular transport carriers that mediate secretion and interorganelle trafficking. Are lipids also sorted into distinct TGN-derived carriers? The Golgi is the principal site of the synthesis of sphingomyelin (SM), an abundant sphingolipid that is transported. To address the specificity of SM transport to the plasma membrane, we engineered a natural SM-binding pore-forming toxin, equinatoxin II (Eqt), into a nontoxic reporter termed Eqt-SM and used it to monitor intracellular trafficking of SM. Using quantitative live cell imaging, we found that Eqt-SM is enriched in a subset of TGN-derived secretory vesicles that are also enriched in a glycophosphatidylinositol-anchored protein. In contrast, an integral membrane secretory protein (CD8α) is not enriched in these carriers. Our results demonstrate the sorting of native SM at the TGN and its transport to the plasma membrane by specific carriers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.