Evidence map›Paper›PMID 27246976›Full record

ArticleOncotarget2017

Long noncoding RNA ZFAS1 promotes gastric cancer cells proliferation by epigenetically repressing KLF2 and NKD2 expression.

Fengqi Nie, Xiang Yu, Mingde Huang, Yunfei Wang, Min Xie, Hongwei Ma, Zhaoxia Wang, Wei De, Ming Sun

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Oncotarget, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 99 papers, 4 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
99citing papers in PubMed, 4 pooled it
11.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

99 citing papers in PubMed, 4 syntheses or guidelines pooled it, 159 citations in OpenAlex.

  1. The regulatory role ofOncology research · 2025
    Pooled it
  2. Pooled it
  3. Pooled it
  4. Long noncoding RNAs as novel serum biomarkers for the diagnosis of hepatocellular carcinoma: a systematic review and meta-analysis.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2017
    Pooled it
  5. Trial
  6. Review
  7. Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Review
  13. Review
  14. Article
  15. Review
  16. Review
  17. Review
  18. Article
  19. Review
  20. Review

39 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 6 institutions in 2 countries.

Fengqi NieDepartment of Oncology, Second Affiliated Hospital, Nanjing Medical University, Nanjing, People's Republic of China.
Xiang YuDepartment of General Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University Medical College, Yantai, People's Republic of China.
Mingde HuangDepartment of Medical Oncology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, People's Republic of China.
Yunfei WangDepartment of Bioinformatics and Computational Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
Min XieDepartment of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, People's Republic of China.
Hongwei MaDepartment of Pathology, First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
Zhaoxia WangDepartment of Oncology, Second Affiliated Hospital, Nanjing Medical University, Nanjing, People's Republic of China.
Wei DeDepartment of Biochemistry and Molecular Biology, Nanjing Medical University, Nanjing, People's Republic of China.
Ming SunDepartment of Bioinformatics and Computational Biology, UT MD Anderson Cancer Center, Houston, TX, USA.
Nanjing Medical University · CNSecond Affiliated Hospital of Nanjing Medical University · CNThe University of Texas MD Anderson Cancer Center · USHuaian First People’s Hospital · CNJiangsu Province Hospital · CNQingdao University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recently, long noncoding RNAs have been emerged as critical regulators of human disease and prognostic markers in several cancers, including gastric cancer. In this study, we globally assessed the transcriptomic differences of lncRNAs in gastric cancer using publicly available microarray data from Gene Expression Omnibus (GEO) and identified an oncogenetic lncRNA ZFAS1, which may promote gastric tumorigenesis. ZFAS1 has been found to be upregulated and function as oncogene in colorectal cancer and hepatocellular carcinoma, but its expression pattern, biological function and underlying mechanism in gastric cancer is still undetermined. Here, we reported that ZFAS1 expression is also overexpressed in gastric cancer, and its increased level is associated with poor prognosis and shorter survival. Knockdown of ZFAS1 impaired gastric cancer cells proliferation and induced apoptosis in vitro, and inhibited tumorigenicity of gastric cancer cells in vivo. Mechanistically, RNA immunoprecipitation and RNA pull-down experiment showed that ZFAS1 could simultaneously interact with EZH2 and LSD1/CoREST to repress underlying targets KLF2 and NKD2 transcription. In addition, rescue experiments determined that ZFAS1 oncogenic function is partly dependent on repressing KLF2 and NKD2. Taken together, our findings illuminate how ZFAS1 over-expression confers an oncogenic function in gastric cancer.

Indexed as

Adaptor Proteins, Signal TransducingAdultAgedAnimalsBiomarkers, TumorCalcium-Binding ProteinsCarcinogenesisCarrier ProteinsCell Line, TumorCell ProliferationEpigenesis, GeneticFemaleGene Expression Regulation, NeoplasticHeterograftsHumansKaplan-Meier EstimateAdaptor Proteins, Signal TransducingBiomarkers, TumorCalcium-Binding ProteinsCarrier ProteinsKLF2 protein, humanKruppel-Like Transcription FactorsNKD2 protein, humanRNA, Long NoncodingZFAS1 long non-coding RNA, humangastric cancerKLF2 and NKD2long noncoding RNAproliferationZFAS1

Identifiers

PMID27246976
PMCPMC5503528
OpenAlexW2403105296

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.