Evidence map›Paper›PMID 27246809›Full record

ArticleIslets2016

Ginsenoside Rg3 prevents INS-1 cell death from intermittent high glucose stress.

You Jeong Kim, Su Min Park, Hye Sook Jung, Eun Ju Lee, Tae Kyoon Kim, Tae-Nyun Kim, Min Jeong Kwon, Soon Hee Lee, Byoung Doo Rhee, Mi-Kyung Kim and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Islets, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 43 citations in OpenAlex.

  1. Review
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  13. Chinese MedicineEvidence-based complementary and alternative medicine : eCAM · 2019
    Article
  14. Advances in Research on the Protective Mechanisms of Traditional Chinese Medicine (TCM) in IsletEvidence-based complementary and alternative medicine : eCAM · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

You Jeong Kima Division of Endocrinology and Metabolism, Department of Internal Medicine, Maryknoll Medical Center , Busan , South Korea.
Su Min Parkb Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Inje University , Busan , South Korea.
Hye Sook Jungc Paik Institute for Clinical Research, Molecular Therapy Lab, Inje University , Busan , South Korea.
Eun Ju Leeb Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Inje University , Busan , South Korea.
Tae Kyoon Kimb Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Inje University , Busan , South Korea.
Tae-Nyun Kimb Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Inje University , Busan , South Korea.
Min Jeong Kwonb Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Inje University , Busan , South Korea.
Soon Hee Leeb Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Inje University , Busan , South Korea.
Byoung Doo Rheeb Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Inje University , Busan , South Korea.
Mi-Kyung Kimb Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Inje University , Busan , South Korea.
Jeong Hyun Parkb Division of Endocrinology and Metabolism, Department of Internal Medicine, College of Medicine, Inje University , Busan , South Korea.
Inje University · KRInje University Busan Paik Hospital · KRMaryknoll Medical Center · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGinsenoside Rg3 has been proposed to mediate anti-diabetic effects, but their direct effect on pancreatic β cell viability and mechanisms are not clearly understood. Recent studies suggest that intermittent high glucose (IHG) could be more harmful to pancreatic β cells than sustained high glucose. There are few reports about the effect of the ginsenosideRg3 to β cell apoptosis and proliferation against IHG.

methodsINS-1 cells were treated with alternative glucose concentration with or without ginsenoside Rg3. Cell apoptosis and viability were detected by Annexin V staining and MTT assay. The activation of mitogen-activated protein kinases (MAPKs) was analyzed by Western blotting using specific antibodies. Quantification of secreted insulin protein was measured using rat/mouse Insulin ELISA kits. Bromodeoxyuridine (BrdU) staining and florescence in situ hybridization (FISH) analysis was performed to compare cell proliferation.

resultINS-1 cell viability was decreased under IHG and increased with Rg3 treatment.Rg3 significantly reduced the apoptotic INS-1 cells against IHG. The quantification of secreted insulin concentration was increased with Rg3. Rg3 increased INS-1 cell proliferation. ERK and p38 MAPK pathways reduced by IHG were activated by the ginsenoside Rg3.

conclusionGinsenoside Rg3 protected INS-1 cell death from IHG with reducing apoptosis and increasing proliferation.

Indexed as

AnimalsAnnexin A5ApoptosisBlotting, WesternCell DeathCells, CulturedCell SurvivalGinsenosidesGlucoseHumansInsulinInsulin-Secreting CellsInsulin SecretionRatsAnnexin A5ginsenoside Rg3GinsenosidesGlucoseInsulinanti-apoptosiscell proliferationcytoprotectionginsenoside Rg3insulin-secreting cells

Identifiers

PMID27246809
PMCPMC4987017
OpenAlexW2418327402

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.