ArticleScientific reports2016
Establishment and application of a real-time loop-mediated isothermal amplification system for the detection of CYP2C19 polymorphisms.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Development of primers for loop-mediated isothermal amplification for Mycoplasma canadense detection.The Journal of veterinary medical science · 2025Article
- A novel AllGlo probe-quantitative PCR method for detecting single nucleotide polymorphism in CYP2C19 to evaluate the antiplatelet activity of clopidogrel.Scientific reports · 2024Article
- Versatility of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) from diagnosis of early pathological infection to mutation detection in organisms.Molecular biology reports · 2024Review
- Diagnosis of α-thalassaemia by colorimetric gap loop mediated isothermal amplification.Scientific reports · 2023Article
- Advances in point-of-care genetic testing for personalized medicine applications.Biomicrofluidics · 2023Review
- Article
- Super-assembly of integrated gold magnetic assay with loop-mediated isothermal amplification for point-of-care testing.Nano research · 2023Article
- Review
- Non-invasive detection of EGFR mutations by cell-free loop-mediated isothermal amplification (CF-LAMP).Scientific reports · 2020Article
- Genotyping on blood and buccal cells using loop-mediated isothermal amplification in healthy humans.Biotechnology reports (Amsterdam, Netherlands) · 2020Article
- Loop Mediated Isothermal Amplification: A Promising Tool for Screening Genetic Mutations.Molecular diagnosis & therapy · 2019Review
- Allele-Specific Isothermal Amplification Method Using Unmodified Self-Stabilizing Competitive Primers.Analytical chemistry · 2018Article
- Genotyping of Multiple Clinical Samples with a Combined Direct PCR and Magnetic Lateral Flow Assay.iScience · 2018Article
- A direct isothermal amplification system adapted for rapid SNP genotyping of multifarious sample types.Biosensors & bioelectronics · 2018Article
- Allele specific LAMP- gold nanoparticle for characterization of single nucleotide polymorphisms.Biotechnology reports (Amsterdam, Netherlands) · 2017Article
- Development of a loop-mediated isothermal amplification technique and comparison with quantitative real-time PCR for the rapid visual detection of canine neosporosis.Parasites & vectors · 2017Article
- Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi.Scientific reports · 2017Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-nucleotide polymorphisms (SNPs) represent the most widespread type of genetic variation (approximately 90%) in the human genome, and the demand to overcome such variation has received more attention now than ever before. The capacity to rapidly assess SNPs that correlate with disease predisposition, drug efficacy and drug toxicity is a key step for the development of personalized medicine. In this work, a rapid one-step SNP detection method, real-time loop-mediated isothermal amplification (RT-LAMP), was first applied for CYP2C19 polymorphisms testing. The optimized method was established with specifically designed primers for target amplification by real-time detection in approximately 30 min under isothermal conditions. RT-LAMP amplified few copies of template to produce significant amounts of product and quantitatively detected human DNA with compatible specificity and sensitivity. The success in the establishment of this RT-LAMP protocol for CYP2C19 polymorphism testing is significant for the extension of this technique for the detection of other SNPs, which will further facilitate the development of personalized medicine.
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