Evidence map›Paper›PMID 27246220›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2016

Sirtuin 6 (SIRT6) Activity Assays.

Minna Rahnasto-Rilla, Maija Lahtela-Kakkonen, Ruin Moaddel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. NADCell communication and signaling : CCS · 2024
    Review
  2. Role of mono-ADP-ribosylation histone modification (Review).Experimental and therapeutic medicine · 2021
    Review
  3. Review
  4. Effects of galloflavin and ellagic acid on sirtuin 6 and its anti-tumorigenic activities.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2020
    Article
  5. Sirtuins and SIRT6 in Carcinogenesis and in Diet.International journal of molecular sciences · 2019
    Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Minna Rahnasto-RillaBioanalytical and Drug Development Unit, National institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD, 21224-6825, USA.
Maija Lahtela-KakkonenSchool of Pharmacy, University of Eastern Finland, Kuopio, 70210, Finland.
Ruin MoaddelBioanalytical and Drug Development Unit, National institute on Aging, National Institutes of Health, 251 Bayview Boulevard, Suite 100, Baltimore, MD, 21224-6825, USA. moaddelru@grc.nia.nih.gov.

Funding

Immobilized Receptors In Drug DiscoveryZIAAG000295 · NIA · NATIONAL INSTITUTE ON AGING · PI EGAN, JOSEPHINE · 2009 to 2025
$11.4M
Intramural NIH HHS Z99 AG999999Intramural NIH HHS ZIA AG000295
6 · The paper itself

Abstract

SIRT6 has been shown to possess weak deacetylation, mono-ADP-ribosyltransferase activity, and deacylation activity in vitro. SIRT6 selectively deacetylates H3K9Ac and H3K56Ac. Several SIRT6 assays have been developed including HPLC assays, fluorogenic assays, FRET, magnetic beads, in silico, and bioaffinity chromatography assays. Herein, we describe detailed protocols for the HPLC based activity/inhibition assays, magnetic beads deacetylation assays, bioaffinity chromatographic assays as well as fluorogenic and in silico assays.

Indexed as

AcetylationChromatography, High Pressure LiquidComputer SimulationFluorescence Resonance Energy TransferHistonesHumansProtein BindingProtein Interaction MappingSirtuinsHistonesSIRT6 protein, humanSirtuinsActivation assaysBioaffinity chromatographyDeacetylation assaysInhibition assaysIn silico screening

Identifiers

PMID27246220
PMCPMC5004593

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.