ArticlePlant physiology2016
The Mediator Complex MED15 Subunit Mediates Activation of Downstream Lipid-Related Genes by the WRINKLED1 Transcription Factor.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 91 citations in OpenAlex.
- Article
- A tree peony INDETERMINATE DOMAIN transcription factor PrIDD7 inhibits seed oil accumulation.Plant cell reports · 2026Article
- From wrinkled seeds to plant oil accumulation networks: the legacy of a Plant Physiology classic.Plant physiology · 2026Review
- Integrated metabolome and transcriptome analysis of castor oil accumulation during seed development inFrontiers in plant science · 2026Article
- Genome-Wide Identification ofPlants (Basel, Switzerland) · 2025Article
- Plant oil biosynthesis and genetic improvement: progress, challenges, and opportunities.Plant physiology · 2025Review
- Genetic suppressors of the growth-immunity trade-off in the Arabidopsis salicylic acid-accumulating dmr6 dlo1 mutant.The Plant journal : for cell and molecular biology · 2025Article
- Article
- DELLA proteins recruit the Mediator complex subunit MED15 to coactivate transcription in land plants.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Progress in understanding and improving oil content and quality in seeds.Frontiers in plant science · 2023Review
- Transcriptional regulation of oil biosynthesis in seed plants: Current understanding, applications, and perspectives.Plant communications · 2022Review
- BLISTER promotes seed maturation and fatty acid biosynthesis by interacting with WRINKLED1 to regulate chromatin dynamics in Arabidopsis.The Plant cell · 2022Article
- An expanded role for the transcription factorFrontiers in plant science · 2022Article
- A genome-scale TF-DNA interaction network of transcriptional regulation of Arabidopsis primary and specialized metabolism.Molecular systems biology · 2021Article
- Small RNA profiling for identification of microRNAs involved in regulation of seed development and lipid biosynthesis in yellowhorn.BMC plant biology · 2021Article
- Mobilizing Vacuolar Sugar Increases Vegetative Triacylglycerol Accumulation.Frontiers in plant science · 2021Article
- MedProDB: A database of Mediator proteins.Computational and structural biotechnology journal · 2021Article
- Differential Activation of Partially Redundant Δ9 Stearoyl-ACP Desaturase Genes Is Critical for Omega-9 Monounsaturated Fatty Acid Biosynthesis During Seed Development in Arabidopsis.The Plant cell · 2020Article
- The function of the WRI1-TCP4 regulatory module in lipid biosynthesis.Plant signaling & behavior · 2020Article
- Lipid Metabolism in Plants.Plants (Basel, Switzerland) · 2020Article
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Mediator complex is known to be a master coordinator of transcription by RNA polymerase II, and this complex is recruited by transcription factors (TFs) to target promoters for gene activation or repression. The plant-specific TF WRINKLED1 (WRI1) activates glycolysis-related and fatty acid biosynthetic genes during embryogenesis. However, no Mediator subunit has yet been identified that mediates WRI1 transcriptional activity. Promoter-β-glucuronidase fusion experiments showed that MEDIATOR15 (MED15) is expressed in the same cells in the embryo as WRI1. We found that the Arabidopsis (Arabidopsis thaliana) MED15 subunit of the Mediator complex interacts directly with WRI1 in the nucleus. Overexpression of MED15 or WRI1 increased transcript levels of WRI1 target genes involved in glycolysis and fatty acid biosynthesis; these genes were down-regulated in wild-type or WRI1-overexpressing plants by silencing of MED15 However, overexpression of MED15 in the wri1 mutant also increased transcript levels of WRI1 target genes, suggesting that MED15 also may act with other TFs to activate downstream lipid-related genes. Chromatin immunoprecipitation assays confirmed the association of MED15 with six WRI1 target gene promoters. Additionally, silencing of MED15 resulted in reduced fatty acid content in seedlings and mature seeds, whereas MED15 overexpression increased fatty acid content in both developmental stages. Similar results were found in wri1 mutant and WRI1 overexpression lines. Together, our results indicate that the WRI1/MED15 complex transcriptionally regulates glycolysis-related and fatty acid biosynthetic genes during embryogenesis.
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