Evidence map›Paper›PMID 27246097›Full record

ArticlePlant physiology2016

Promises and Challenges of Eco-Physiological Genomics in the Field: Tests of Drought Responses in Switchgrass.

John T Lovell, Eugene V Shakirov, Scott Schwartz, David B Lowry, Michael J Aspinwall, Samuel H Taylor, Jason Bonnette, Juan Diego Palacio-Mejia, Christine V Hawkes, Philip A Fay and 1 more

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Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed.

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  13. Metabolite and transcript profiling of Guinea grass (Panicum maximum Jacq) response to elevated [COMetabolomics : Official journal of the Metabolomic Society · 2019
    Article
  14. Review
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  20. Focus on Ecophysiology.Plant physiology · 2016
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

John T LovellDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.).ORCID 0000-0002-8938-1166
Eugene V ShakirovDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.).ORCID 0000-0003-2689-7410
Scott SchwartzDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.).
David B LowryDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.).
Michael J AspinwallDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.).
Samuel H TaylorDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.).ORCID 0000-0001-9714-0656
Jason BonnetteDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.).ORCID 0000-0003-1835-8409
Juan Diego Palacio-MejiaDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.).
Christine V HawkesDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.).
Philip A FayDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.).
Thomas E JuengerDepartment of Integrative Biology, University of Texas at Austin, Austin, Texas (J.T.L., E.V.S., S.S., J.B., J.D.P.-M., C.V.H., T.E.J.); Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Republic of Tatarstan, Russia (E.V.S.); Department of Plant Biology, Michigan State University, East Lansing, Michigan (D.B.L.);Hawkesbury Institute for the Environment, Western Sydney University, Richmond, Australia (M.J.A.); Departments of Environmental Studies and Biology, Keene State College, Keene, New Hampshire USA (S.H.T.); andUSDA-ARS Grassland Soil and Water Research Laboratory, Temple, Texas 76502 (P.A.F.) tjuenger@austin.utexas.edu.ORCID 0000-0001-9550-9288

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Identifying the physiological and genetic basis of stress tolerance in plants has proven to be critical to understanding adaptation in both agricultural and natural systems. However, many discoveries were initially made in the controlled conditions of greenhouses or laboratories, not in the field. To test the comparability of drought responses across field and greenhouse environments, we undertook three independent experiments using the switchgrass reference genotype Alamo AP13. We analyzed physiological and gene expression variation across four locations, two sampling times, and three years. Relatively similar physiological responses and expression coefficients of variation across experiments masked highly dissimilar gene expression responses to drought. Critically, a drought experiment utilizing small pots in the greenhouse elicited nearly identical physiological changes as an experiment conducted in the field, but an order of magnitude more differentially expressed genes. However, we were able to define a suite of several hundred genes that were differentially expressed across all experiments. This list was strongly enriched in photosynthesis, water status, and reactive oxygen species responsive genes. The strong across-experiment correlations between physiological plasticity-but not differential gene expression-highlight the complex and diverse genetic mechanisms that can produce phenotypically similar responses to various soil water deficits.

Indexed as

DroughtsGene Expression Regulation, PlantAdaptation, PhysiologicalEcosystemGene Expression ProfilingGenomicsPanicumPhotosynthesisPlant LeavesPrincipal Component AnalysisSoilStress, PhysiologicalWaterSoilWater

Identifiers

PMID27246097
PMCPMC5047078

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.