ArticleRNA biology2016
Reduced stability and intracellular transport of dsRNA contribute to poor RNAi response in lepidopteran insects.
Article in RNA biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
What it found
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Who cites it
111 citing papers in PubMed, 293 citations in OpenAlex.
- Advancing the adoption of RNA interference-based biopesticides.Nature plants · 2026Review
- Review
- Optimization of dsRNA Length and Target Position for Enhanced RNA Interference Efficiency Targeting the Chitinase GeneInsects · 2026Article
- RNAi: the next wave of green agriculture, challenges, and bridging the translational gap.Plant cell reports · 2026Review
- Emerging experimental and bioinformatic approaches in RNA interference-based pest control research.Insect molecular biology · 2026Review
- RNAi Power Targets in Insect Pests: Beyond Functional Validation to Biopesticide Development Potential.Plants (Basel, Switzerland) · 2026Review
- Article
- Evaluating the potential of RNA interference for control of striped cucumber beetle, Acalymma vittatum (Fabricius) (Coleoptera: Chrysomelidae).Pest management science · 2026Article
- TAV2b Peptide Derivatives Underwind and Stabilize Double-Stranded RNA upon Binding.Journal of the American Chemical Society · 2026Article
- Next-generation insect pest management: genetic innovations and emerging biocontrol strategies.Frontiers in plant science · 2026Review
- Improving RNAi in the fall armyworm,Frontiers in insect science · 2026Article
- Overview of polymeric polyplexes for dsRNA delivery in insects: complexation, stability, and design considerations.Frontiers in insect science · 2026Review
- Enhancing the delivery and stability of lipid nanoparticle-dsRNA formulations in the RNAi-recalcitrant fall armyworm (Frontiers in insect science · 2026Article
- RNAi ofInsects · 2025Article
- Double-Stranded DNA Reduces dsRNA Degradation in the Saliva and Significantly Enhanced RNAi-Mediated Gene Silencing in Halyomorpha halys.Advanced biology · 2025Article
- Efficient In Vitro Plantlet Regeneration from Stolon Explants and Genetic Stability Assessment Using ISSR Markers in the Ornamental FernPlants (Basel, Switzerland) · 2025Article
- The quest for the best target genes for RNAi-mediated pest control.Insect molecular biology · 2025Review
- Recent advances in understanding of the mechanisms of RNA interference in insects.Insect molecular biology · 2025Review
- Review
- The functional of cathepsin L in the ontogenesis and reproductive regulation of the Asian corn borer, Ostrinia furnacalis.Scientific reports · 2025Article
51 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference (RNAi) has become a widely used reverse genetic tool to study gene function in eukaryotic organisms and is being developed as a technology for insect pest management. The efficiency of RNAi varies among organisms. Insects from different orders also display differential efficiency of RNAi, ranging from highly efficient (coleopterans) to very low efficient (lepidopterans). We investigated the reasons for varying RNAi efficiency between lepidopteran and coleopteran cell lines and also between the Colorado potato beetle, Leptinotarsa decemlineata and tobacco budworm, Heliothis virescens. The dsRNA either injected or fed was degraded faster in H. virescens than in L. decemlineata. Both lepidopteran and coleopteran cell lines and tissues efficiently took up the dsRNA. Interestingly, the dsRNA administered to coleopteran cell lines and tissues was taken up and processed to siRNA whereas the dsRNA was taken up by lepidopteran cell lines and tissues but no siRNA was detected in the total RNA isolated from these cell lines and tissues. The data included in this paper showed that the degradation and intracellular transport of dsRNA are the major factors responsible for reduced RNAi efficiency in lepidopteran insects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.