ArticleeLife2016
Viral hijacking of a replicative helicase loader and its implications for helicase loading control and phage replication.
Article in eLife, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Novel phage infecting themSphere · 2024Article
- Phage protein Gp11 blocksmBio · 2024Article
- Frequent nonhomologous replacement of replicative helicase loaders by viruses inProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Non-canonical Staphylococcus aureus pathogenicity island repression.Nucleic acids research · 2022Article
- Diverse ATPase Proteins in Mobilomes Constitute a Large Potential Sink for Prokaryotic Host ATP.Frontiers in microbiology · 2021Article
- Genome replication dynamics of a bacteriophage and its satellite reveal strategies for parasitism and viral restriction.Nucleic acids research · 2020Article
- Exploring the whole standard operating procedure for phage therapy in clinical practice.Journal of translational medicine · 2019Review
- Virus-host protein-protein interactions of mycobacteriophage Giles.Scientific reports · 2017Article
- Convergent evolution involving dimeric and trimeric dUTPases in pathogenicity island mobilization.PLoS pathogens · 2017Article
- Sak and Sak4 recombinases are required for bacteriophage replication in Staphylococcus aureus.Nucleic acids research · 2017Article
- Transcriptomic and Metabolomics Profiling of Phage-Host Interactions between Phage PaP1 andFrontiers in microbiology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Replisome assembly requires the loading of replicative hexameric helicases onto origins by AAA+ ATPases. How loader activity is appropriately controlled remains unclear. Here, we use structural and biochemical analyses to establish how an antimicrobial phage protein interferes with the function of the Staphylococcus aureus replicative helicase loader, DnaI. The viral protein binds to the loader's AAA+ ATPase domain, allowing binding of the host replicative helicase but impeding loader self-assembly and ATPase activity. Close inspection of the complex highlights an unexpected locus for the binding of an interdomain linker element in DnaI/DnaC-family proteins. We find that the inhibitor protein is genetically coupled to a phage-encoded homolog of the bacterial helicase loader, which we show binds to the host helicase but not to the inhibitor itself. These findings establish a new approach by which viruses can hijack host replication processes and explain how loader activity is internally regulated to prevent aberrant auto-association.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.