ArticleACS chemical biology2016
A Novel Destabilizing Domain Based on a Small-Molecule Dependent Fluorophore.
Article in ACS chemical biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Time-Resolved Analysis of Protein-Protein Ensembles Using a Destabilizing Domain to Map Dynamic Interactions of SARS-CoV-2 nsp15.ACS chemical biology · 2025Article
- The HELP-UnaG Fusion Protein as a Bilirubin Biosensor: From Theory to Mature Technological Development.Molecules (Basel, Switzerland) · 2025Review
- Engineering stringent genetic biocontainment of yeast with a protein stability switch.Nature communications · 2024Article
- Synthetic genetic circuits to uncover the OCT4 trajectories of successful reprogramming of human fibroblasts.Science advances · 2023Article
- Adaptable, turn-on maturation (ATOM) fluorescent biosensors for multiplexed detection in cells.Nature methods · 2023Article
- Fast friends - Ubiquitin-like modifiers as engineered fusion partners.Seminars in cell & developmental biology · 2022Review
- A cAMP Sensor Based on Ligand-Dependent Protein Stabilization.ACS chemical biology · 2022Article
- A Method for Conditional Regulation of Protein Stability in Native or Near-Native Form.Cell chemical biology · 2020Article
- Applying Antibodies Inside Cells: Principles and Recent Advances in Neurobiology, Virology and Oncology.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2020Review
- Bright ligand-activatable fluorescent protein for high-quality multicolor live-cell super-resolution microscopy.Nature communications · 2020Article
- Advances in targeted degradation of endogenous proteins.Cellular and molecular life sciences : CMLS · 2019Review
- A Chemical Biology View of Bioactive Small Molecules and a Binder-Based Approach to Connect Biology to Precision Medicines.Israel journal of chemistry · 2019Article
- A Drug-Tunable Gene Therapy for Broad-Spectrum Protection against Retinal Degeneration.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- The development of a sensitive fluorescent protein-based transcript reporter for high throughput screening of negative modulators of lncRNAs.Genes & diseases · 2018Article
- Live-cell imaging of cell signaling using genetically encoded fluorescent reporters.The FEBS journal · 2018Review
- The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins.Trends in biochemical sciences · 2017Review
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Tools that can directly regulate the activity of any protein-of-interest are valuable in the study of complex biological processes. Herein, we describe the development of a novel protein domain that exhibits small molecule-dependent stability and fluorescence based on the bilirubin-inducible fluorescent protein, UnaG. When genetically fused to any protein-of-interest, this fluorescent destabilizing domain (FDD) confers its instability to the entire fusion protein, facilitating the rapid degradation of the fusion. In the presence of its cognate ligand bilirubin (BR), the FDD fusion becomes stable and fluorescent. This new chemical genetic tool allows for rapid, reversible, and tunable control over the stability and fluorescence of a wide range of protein targets.
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Registered trials
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