ArticleScientific reports2016
The Statistical Value of Raw Fluorescence Signal in Luminex xMAP Based Multiplex Immunoassays.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 79 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
79 citing papers in PubMed.
- Landscape of coordinated immune responses to H1N1 challenge in humans.The Journal of clinical investigation · 2020Trial
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- Evaluation of Human Stem Cell Conditioned Medium and Culture Conditions on Alleviating Elastase-Compromised Human Aortic Smooth Muscle Cell Function.Cardiovascular engineering and technology · 2026Article
- Association Between Inflammatory Biomarkers And Carotid Ultrasound Parameters In The Northern Manhattan Stroke Study.Translational stroke research · 2026Article
- Immune Factors Linked to Long-Term HCV Humoral Memory Five Years After Cure in People with HIV: A Cross-Sectional Study.Pharmaceuticals (Basel, Switzerland) · 2026Article
- HIV status defines distinct immunological drivers of persistent portal hypertension after HCV cure in people with advanced cirrhosis.Frontiers in immunology · 2026Article
- IFNL4-rs12979860 CC genotype predisposes to accelerated terminal exhaustion and senescence in HIV/HCV-chronic infection.Journal of translational medicine · 2025Article
- Heritability of neonatal acute phase protein levels.Brain, behavior, & immunity - health · 2025Article
- The effects of the COVID-19 pandemic on neurobiological functioning in adolescents.Translational psychiatry · 2025Article
- Increased Colonic Levels of CD8+ Cytotoxic T lymphocyte-Associated Mediators in Patients With Microscopic Colitis.Inflammatory bowel diseases · 2025Article
- Measuring cytokines in Eurasian tundra reindeer (Rangifer tarandus tarandus) with a bovine bead-based multiplex immunoassay and real-time PCR.Acta veterinaria Scandinavica · 2025Article
- Impact of HCV elimination on metabolism markers in people living with HIV.Scientific reports · 2025Article
- Article
- Association of a multiplex immune marker panel with incident cognitive impairment and dementia: The Northern Manhattan Study.Brain, behavior, & immunity - health · 2025Article
- Value-based cognitive control moderates the relation of inflammation with depression in adolescents.Social cognitive and affective neuroscience · 2025Article
- Gastrointestinal permeability and kidney injury risk during hyperthermia in young and older adults.Experimental physiology · 2025Article
- Inflammatory markers in pregnancy - identifying drivers in four large cohorts.Frontiers in immunology · 2025Article
- Immunological correlates of suicidality among adolescents with internalizing symptoms.Brain, behavior, & immunity - health · 2024Article
- Beyond basic characterization and omics: Immunomodulatory roles of platelet-derived extracellular vesicles unveiled by functional testing.Journal of extracellular vesicles · 2024Article
- Socioeconomic Disadvantage Moderates the Association of Systemic Inflammation With Amygdala Volume in Adolescents Over a 2-Year Interval: An Exploratory Study.Biological psychiatry. Cognitive neuroscience and neuroimaging · 2024Article
19 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tissue samples (plasma, saliva, serum or urine) from 169 patients classified as either normal or having one of seven possible diseases are analysed across three 96-well plates for the presences of 37 analytes using cytokine inflammation multiplexed immunoassay panels. Censoring for concentration data caused problems for analysis of the low abundant analytes. Using fluorescence analysis over concentration based analysis allowed analysis of these low abundant analytes. Mixed-effects analysis on the resulting fluorescence and concentration responses reveals a combination of censoring and mapping the fluorescence responses to concentration values, through a 5PL curve, changed observed analyte concentrations. Simulation verifies this, by showing a dependence on the mean florescence response and its distribution on the observed analyte concentration levels. Differences from normality, in the fluorescence responses, can lead to differences in concentration estimates and unreliable probabilities for treatment effects. It is seen that when fluorescence responses are normally distributed, probabilities of treatment effects for fluorescence based t-tests has greater statistical power than the same probabilities from concentration based t-tests. We add evidence that the fluorescence response, unlike concentration values, doesn't require censoring and we show with respect to differential analysis on the fluorescence responses that background correction is not required.
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