Evidence map›Paper›PMID 27242995›Full record

ArticleFrontiers in chemistry2016

Improved Tracking and Resolution of Bacteria in Holographic Microscopy Using Dye and Fluorescent Protein Labeling.

Jay L Nadeau, Yong Bin Cho, Jonas Kühn, Kurt Liewer

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 20 citations in OpenAlex.

  1. Viability and Motility ofLife (Basel, Switzerland) · 2024
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 3 countries.

Jay L NadeauGraduate Aerospace Laboratories, California Institute of TechnologyPasadena, CA, USA; Department of Biomedical Engineering, McGill UniversityMontreal, QC, Canada.
Yong Bin ChoGraduate Aerospace Laboratories, California Institute of TechnologyPasadena, CA, USA; Department of Biomedical Engineering, McGill UniversityMontreal, QC, Canada.
Jonas KühnGraduate Aerospace Laboratories, California Institute of TechnologyPasadena, CA, USA; Institute for Astronomy, ETH ZürichZürich, Switzerland.
Kurt LiewerJet Propulsion Laboratory, California Institute of Technology Pasadena, CA, USA.
California Institute of Technology · USJet Propulsion Laboratory · USMcGill University · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Digital holographic microscopy (DHM) is an emerging imaging technique that permits instantaneous capture of a relatively large sample volume. However, large volumes usually come at the expense of lower spatial resolution, and the technique has rarely been used with prokaryotic cells due to their small size and low contrast. In this paper we demonstrate the use of a Mach-Zehnder dual-beam instrument for imaging of labeled and unlabeled bacteria and microalgae. Spatial resolution of 0.3 μm is achieved, providing a sampling of several pixels across a typical prokaryotic cell. Both cellular motility and morphology are readily recorded. The use of dyes provides both amplitude and phase contrast improvement and is of use to identify cells in dense samples.

Indexed as

bacterial motilitybacterial trackingdigital holographic microscopygreen fluorescent proteinsinterferometric microscopyMach-Zehnderquantitative phase imaging

Identifiers

PMID27242995
PMCPMC4874365
OpenAlexW2340252305

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.