ArticleFrontiers in plant science2016
Crystal Structure of Hyp-1, a Hypericum perforatum PR-10 Protein, in Complex with Melatonin.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
- Transgenic validation of a promoter strongly inducible by Agrobacterium tumefaciens.Scientific reports · 2025Article
- Protection of naringenin chalcone by a pathogenesis-related 10 protein promotes flavonoid biosynthesis in Marchantia polymorpha.The New phytologist · 2025Article
- Diverse Therapeutic Potentials of Hypericin: An In-Depth Review.Current topics in medicinal chemistry · 2025Review
- Regulation of Plant Growth and Development by Melatonin.Life (Basel, Switzerland) · 2024Review
- Role of Neurotransmitters (Biomediators) in Plant Responses to Stress.Plants (Basel, Switzerland) · 2024Review
- Why did glutamate, GABA, and melatonin become intercellular signalling molecules in plants?eLife · 2023Review
- A cysteine-rich secretory protein involves in phytohormone melatonin mediated plant resistance to CGMMV.BMC plant biology · 2023Article
- Alkaloid binding to opium poppy major latex proteins triggers structural modification and functional aggregation.Nature communications · 2022Article
- Mammalian Melatonin Agonist Pharmaceuticals Stimulate Rhomboid Proteins in Plants.Biomolecules · 2022Article
- A Systematic Review of Melatonin in Plants: An Example of Evolution of Literature.Frontiers in plant science · 2021Review
- Review
- MALDI-HRMS Imaging Maps the Localization of Skyrin, the Precursor of Hypericin, and Pathway Intermediates in Leaves ofMolecules (Basel, Switzerland) · 2020Article
- A new modulated crystal structure of the ANS complex of the St John's wort Hyp-1 protein with 36 protein molecules in the asymmetric unit of the supercell.Acta crystallographica. Section D, Structural biology · 2020Article
- Ligand Binding of PR-10 Proteins with a Particular Focus on the Bet v 1 Allergen Family.Current allergy and asthma reports · 2020Review
- Nitrogen nutrition modulates oxidative stress and metabolite production in Hypericum perforatum.Protoplasma · 2020Article
- Article
- Purification and Characterization of Pathogenesis Related Class 10 Panallergens.Foods (Basel, Switzerland) · 2019Article
- Auxin driven indoleamine biosynthesis and the role of tryptophan as an inductive signal in Hypericum perforatum (L.).PloS one · 2019Article
- Melatonin Target Proteins: Too Many or Not Enough?Frontiers in endocrinology · 2019Review
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
Hyp-1, a PR-10-fold protein from Hypericum perforatum, was crystallized in complex with melatonin (MEL). The structure confirms the conserved protein fold and the presence of three unusual ligand binding sites, two of which are internal chambers (1,2), while the third one (3) is formed as an invagination of the protein surface. The MEL ligand in site 1 is well defined while that in site 3 seems to be rotating between the side chains of Lys33 and Tyr150 that act as a molecular vise. The patch of electron density in site 2 does not allow unambiguous modeling of a melatonin molecule but suggests a possible presence of its degradation product. This pattern of ligand occupation is reproducible in repeated crystallization/structure determination experiments. Although the binding of melatonin by Hyp-1 does not appear to be very strong (for example, MEL cannot displace the artificial fluorescence probe ANS), it is strong enough to suggest a physiological role of this interaction. For example, trans-zeatin, which is a common ligand of PR-10 proteins, does not overcompete melatonin for binding to Hyp-1 as it does not affect the crystallization process of the Hyp-1/MEL complex, and among a number of potential natural mediators tested, melatonin was the only one to form a crystalline complex with Hyp-1 with the use of standard crystallization screens. Hyp-1 is the second protein in the Protein Data Bank for which melatonin binding has been demonstrated crystallographically, the first one being human quinone reductase.
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