ArticleFrontiers in plant science2016
Isolation and Characterization of Three New Monoterpene Synthases from Artemisia annua.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Discovery and Biosynthesis of Farnesyl Pyrophosphate-Derived Noncanonical CJournal of the American Chemical Society · 2026Article
- Isolation and Characterization of Two Monoterpene Synthases and a Sesquiterpene Synthase fromMetabolites · 2025Article
- The Beneficial Use ofAnimals : an open access journal from MDPI · 2025Review
- Traditional uses, Phytochemistry, Pharmacology, and Toxicology of the GenusCurrent topics in medicinal chemistry · 2024Review
- Genome ofiScience · 2022Article
- Light Intensity and Temperature Effect onFrontiers in plant science · 2022Article
- Kinetic studies and homology modeling of a dual-substrate linalool/nerolidol synthase from Plectranthus amboinicus.Scientific reports · 2021Article
- Plasticity engineering of plant monoterpene synthases and application for microbial production of monoterpenoids.Biotechnology for biofuels · 2021Review
- Artemisia annua L. and photoresponse: from artemisinin accumulation, volatile profile and anatomical modifications to gene expression.Plant cell reports · 2020Article
- Untargeted Metabolomics Approach Reveals Diverse Responses ofMetabolites · 2019Article
- The occurrence and formation of monoterpenes in herbivore-damaged poplar roots.Scientific reports · 2018Article
- Use of a temporary immersion bioreactor system for the sustainable production of thapsigargin in shoot cultures ofPlant methods · 2018Article
- Article
- New insights into artemisinin regulation.Plant signaling & behavior · 2017Review
- Structural and Chemical Biology of Terpenoid Cyclases.Chemical reviews · 2017Review
- Identification of a Novel (-)-5-Epieremophilene Synthase fromFrontiers in plant science · 2017Article
- A Systematic Review of Anti-malarial Properties, Immunosuppressive Properties, Anti-inflammatory Properties, and Anti-cancer Properties of Artemisia Annua.Electronic physician · 2016Review
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Artemisia annua, an annual herb used in traditional Chinese medicine, produces a wealth of monoterpenes and sesquiterpenes, including the well-known sesquiterpene lactone artemisinin, an active ingredient in the treatment for malaria. Here we report three new monoterpene synthases of A. annua. From a glandular trichome cDNA library, monoterpene synthases of AaTPS2, AaTPS5, and AaTPS6, were isolated and characterized. The recombinant proteins of AaTPS5 and AaTPS6 produced multiple products with camphene and 1,8-cineole as major products, respectively, and AaTPS2 produced a single product, β-myrcene. Although both Mg(2+) and Mn(2+) were able to support their catalytic activities, altered product spectrum was observed in the presence of Mn(2+) for AaTPS2 and AaTPS5. Analysis of extracts of aerial tissues and root of A. annua with gas chromatography-mass spectrometry detected more than 20 monoterpenes, of which the three enzymes constituted more than 1/3 of the total. Mechanical wounding induced the expression of all three monoterpene synthase genes, and transcript levels of AaTPS5 and AaTPS6 were also elevated after treatments with phytohormones of methyl jasmonate, salicylic acid, and gibberellin, suggesting a role of these monoterpene synthases in plant-environment interactions. The three new monoterpene synthases reported here further our understanding of molecular basis of monoterpene biosynthesis and regulation in plant.
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