ArticleFrontiers in microbiology2016
Proteomic Analysis of a Novel Bacillus Jumbo Phage Revealing Glycoside Hydrolase As Structural Component.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
- Phage anti-defense genes targeting NADVirulence · 2026Article
- Multiomics Approaches for Bacteriophage-Based Biocontrol Applications: A Review of Metabolomics, Transcriptomics, and Proteomics.BioMed research international · 2026Review
- Proteomic Analysis of Marine Bacteriophages: Structural Conservation, Post-Translational Modifications, and Phage-Host Interactions.Environmental microbiology · 2025Article
- Article
- Lysins as a powerful alternative to combat Bacillus anthracis.Applied microbiology and biotechnology · 2024Review
- PhageScanner: a reconfigurable machine learning framework for bacteriophage genomic and metagenomic feature annotation.Frontiers in microbiology · 2024Article
- Novel pelagiphage isolate Polarivirus skadi is a polar specialist that dominates SAR11-associated bacteriophage communities at high latitudes.The ISME journal · 2023Article
- Metaviromic analyses of DNA virus community from sediments of the N-Choe stream, North India.Virus research · 2023Article
- Prediction of Phage Virion Proteins Using Machine Learning Methods.Molecules (Basel, Switzerland) · 2023Article
- Viral Complexity.Biomolecules · 2022Review
- Medium optimization to analyze the protein composition of Bacillus pumilus HR10 antagonizing Sphaeropsis sapinea.AMB Express · 2022Article
- SCORPION is a stacking-based ensemble learning framework for accurate prediction of phage virion proteins.Scientific reports · 2022Article
- Isolation and characterization of two homolog phages infectingFrontiers in microbiology · 2022Article
- Large-scale comparative review and assessment of computational methods for phage virion proteins identification.EXCLI journal · 2022Review
- Ensemble Learning-Based Feature Selection for Phage Protein Prediction.Frontiers in microbiology · 2022Article
- Comparative Genomics of Three Novel Jumbo Bacteriophages Infecting Staphylococcus aureus.Journal of virology · 2021Article
- Pseudo-188D: Phage Protein Prediction Based on a Model of Pseudo-188D.Frontiers in genetics · 2021Article
- Intracellular Organization by Jumbo Bacteriophages.Journal of bacteriology · 2020Review
- Meta-iPVP: a sequence-based meta-predictor for improving the prediction of phage virion proteins using effective feature representation.Journal of computer-aided molecular design · 2020Article
- Multisubunit RNA Polymerases of Jumbo Bacteriophages.Viruses · 2020Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tailed phages with genomes of larger than 200 kbp are classified as Jumbo phages and exhibited extremely high uncharted diversity. The genomic annotation of Jumbo phage is often disappointing because most of the predicted proteins, including structural proteins, failed to make good hits to the sequences in the databases. In this study, 23 proteins of a novel Bacillus Jumbo phage, vB_BpuM_BpSp, were identified as phage structural proteins by the structural proteome analysis, including 14 proteins of unknown function, 5 proteins with predicted function as structural proteins, a glycoside hydrolase, a Holliday junction resolvase, a RNA-polymerase β-subunit, and a host-coding portal protein, which might be hijacked from the host strain during phage virion assembly. The glycoside hydrolase (Gp255) was identified as phage virion component and was found to interact with the phage baseplate protein. Gp255 shows specific lytic activity against the phage host strain GR8 and has high temperature tolerance. In situ peptidoglycan-hydrolyzing activities analysis revealed that the expressed Gp255 and phage structural proteome exhibited glycoside hydrolysis activity against the tested GR8 cell extracts. This study identified the first functional individual structural glycoside hydrolase in phage virion. The presence of activated glycoside hydrolase in phage virions might facilitate the injection of the phage genome during infection by forming pores on the bacterial cell wall.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.