ArticleFrontiers in microbiology2016
Genomic-Based Restriction Enzyme Selection for Specific Detection of Piscirickettsia salmonis by 16S rDNA PCR-RFLP.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Article
- Mobile-CRISPRi as a tool for genetic manipulation in the intracellular pathogenApplied and environmental microbiology · 2026Article
- Transferrin knockout reveals a tolerance phenotype against Piscirickettsia salmonis in Atlantic salmon phagocytes.Veterinary research · 2025Article
- Pathogenomic Insights intoAnimals : an open access journal from MDPI · 2025Article
- Salmonid Rickettsial Septicemia (SRS) disease dynamics and Atlantic salmon immune response to Piscirickettsia salmonis LF-89 and EM-90 co-infection.Veterinary research · 2024Article
- Co-Infection by LF-89-Like and EM-90-Like Genogroups ofPathogens (Basel, Switzerland) · 2023Article
- Cohabitation ofFrontiers in cellular and infection microbiology · 2023Article
- Why DoesFrontiers in immunology · 2022Review
- Development of a Multiplex PCR Assay for Genotyping the Fish PathogenFrontiers in microbiology · 2021Article
- Global Proteomic Profiling ofMicroorganisms · 2020Article
- Pharmacological iron-chelation as an assisted nutritional immunity strategy against Piscirickettsia salmonis infection.Veterinary research · 2020Article
- PCR-RFLP Detection and Genogroup Identification ofPathogens (Basel, Switzerland) · 2020Article
- Article
- Article
- Transcriptomic Changes ofFrontiers in cellular and infection microbiology · 2019Article
- Comparative Genomic Analysis of Three Salmonid Species Identifies Functional Candidate Genes Involved in Resistance to the Intracellular BacteriumFrontiers in genetics · 2019Article
- Analysis ofFrontiers in microbiology · 2017Article
- Comparative Pan-Genome Analysis ofFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gram negative facultative bacterium P. salmonis is the etiological agent of Salmonid Rickettsial Septicaemia (SRS), a severe disease that causes important economic losses in the global salmon farmer industry. Despite efforts to control this disease, the high frequency of new epizootic events indicate that the vaccine and antibiotics treatments have limited effectiveness, therefore the preventive and diagnostic approaches must be improved. A comparison of several methodologies for SRS diagnostic indicate differences in their specificity and its capacity to detect other bacteria coexisting with P. salmonis in culture media (contamination) and fish samples (coinfection), aspects relevant for research, vaccine development and clinical diagnostic. By computer-simulation analyses, we identified a group of restriction enzymes that generate unique P. salmonis 16S rDNA band patterns, distinguishable from all other bacteria. From this information, we designed and developed a PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) assay, which was validated using 16S rDNA universal primers and restriction enzyme PmaCI for the amplification and digestion, respectively. Experimental validation was performed by comparing the restriction pattern of P. salmonis with the restriction patterns generated by bacteria that cohabit with P. salmonis (fish bacterial isolates and culture media contaminants). Our results indicate that the restriction enzyme selection pipeline was suitable to design a more specific, sensible, faster and cheaper assay than the currently used P. salmonis detection methodologies.
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