ArticleThe Journal of cell biology2016
Concentrating pre-mRNA processing factors in the histone locus body facilitates efficient histone mRNA biogenesis.
Article in The Journal of cell biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 102 citations in OpenAlex.
- Cycling off: a novel mechanism for differential cell cycle-dependent histone gene expression.Genes & development · 2026Article
- Are components of the histone gene expression machinery functionally repurposed in terminally differentiated cells?Biology open · 2026Article
- A Novel Mechanism for Autoantigenicity: Condensate Conformational Change.Biomolecules · 2026Article
- Phase separation induced by active polymerization makes protocells robust against environmental changes.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Differential control of both cell cycle-regulated and quantitative histone mRNA expression bybioRxiv : the preprint server for biology · 2026Article
- Precise control of transcription condensates across S phase balances linker histone expression with DNA replication, ensuring genome stability.Molecular cell · 2026Article
- Proximal proteomics reveals a landscape of human nuclear condensates.Nature cell biology · 2025Article
- SUMO2 promotes histone pre-mRNA processing by stabilizing histone locus body interactions and facilitating U7 snRNP assembly.Genes & development · 2025Article
- RNA-associated nuclear condensates: Where the nucleus keeps its RNAs in check.Molecules and cells · 2025Review
- Cell-cycle-regulated transcriptional pausing ofMolecular biology of the cell · 2025Article
- Zelda is dispensable forMolecular biology of the cell · 2025Article
- KPNA3 regulates histone locus body formation by modulating condensation and nuclear import of NPAT.The Journal of cell biology · 2025Article
- Heterogeneity of Molecular Crowding and Liquid-Liquid Phase Separation.Sub-cellular biochemistry · 2025Review
- Cell cycle-regulated transcriptional pausing ofbioRxiv : the preprint server for biology · 2024Article
- Spatial Control over Reactions via Localized Transcription within Membraneless DNA Nanostar Droplets.Journal of the American Chemical Society · 2024Article
- Nuclear RNA: a transcription-dependent regulator of chromatin structure.Biochemical Society transactions · 2024Review
- Article
- Sequence reliance of abioRxiv : the preprint server for biology · 2023Article
- CRISPR-assisted transcription activation by phase-separation proteins.Protein & cell · 2023Article
- Histone locus bodies: a paradigm for how nuclear biomolecular condensates control cell cycle regulated gene expression.Nucleus (Austin, Tex.) · 2023Review
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The histone locus body (HLB) assembles at replication-dependent histone genes and concentrates factors required for histone messenger RNA (mRNA) biosynthesis. FLASH (Flice-associated huge protein) and U7 small nuclear RNP (snRNP) are HLB components that participate in 3' processing of the nonpolyadenylated histone mRNAs by recruiting the endonuclease CPSF-73 to histone pre-mRNA. Using transgenes to complement a FLASH mutant, we show that distinct domains of FLASH involved in U7 snRNP binding, histone pre-mRNA cleavage, and HLB localization are all required for proper FLASH function in vivo. By genetically manipulating HLB composition using mutations in FLASH, mutations in the HLB assembly factor Mxc, or depletion of the variant histone H2aV, we find that failure to concentrate FLASH and/or U7 snRNP in the HLB impairs histone pre-mRNA processing. This failure results in accumulation of small amounts of polyadenylated histone mRNA and nascent read-through transcripts at the histone locus. Thus, the HLB concentrates FLASH and U7 snRNP, promoting efficient histone mRNA biosynthesis and coupling 3' end processing with transcription termination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.