ArticleGenes2016
Selection and Verification of Candidate Reference Genes for Mature MicroRNA Expression by Quantitative RT-PCR in the Tea Plant (Camellia sinensis).
Article in Genes, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Selection of reference genes for normalization of mitochondrial gene expression by qRT-PCR in different potato tissues and during anther development.BMC plant biology · 2025Article
- Comprehensive Profiling of the miRNome and Degradome Reveals Regulatory Signatures of Seed Aging and Germination.International journal of molecular sciences · 2025Article
- From juvenile to adult: investigating miRNAs, gene expression, and the juvenile cone in olive development.Frontiers in plant science · 2025Article
- Screening and validation of optimal miRNA reference genes in different developing stages and tissues of Lilium henryi Baker.Scientific reports · 2024Article
- An Integrated Analysis of microRNAs and the Transcriptome Reveals the Molecular Mechanisms Underlying the Regulation of Leaf Development in Xinyang Maojian Green Tea (Plants (Basel, Switzerland) · 2023Article
- Selection and Optimization of Reference Genes for MicroRNA Expression Normalization by qRT-PCR in Chinese Cedar (International journal of molecular sciences · 2021Article
- Tonoplast-Localized Theanine Transporter CsCAT2 May Mediate Theanine Storage in the Root of Tea Plants (Frontiers in plant science · 2021Article
- Gene Correlation Guided Gene Selection for Microarray Data Classification.BioMed research international · 2021Article
- Argonaute4 Modulates Resistance toPlant physiology · 2020Article
- Utilization of microRNAs and their regulatory functions for improving biotic stress tolerance in tea plant [RNA biology · 2020Article
- Agave angustifolia albino plantlets lose stomatal physiology function by changing the development of the stomatal complex due to a molecular disruption.Molecular genetics and genomics : MGG · 2020Article
- Article
- Identification of Appropriate Reference Genes for Normalizing miRNA Expression in Citrus Infected byGenes · 2019Article
- MicroRNAome Profile ofInternational journal of molecular sciences · 2019Article
- Selection and validation of reference genes for quantitative expression analysis of miRNAs and mRNAs in Poplar.Plant methods · 2019Article
- Selection of reference genes for miRNA qRT-PCR under abiotic stress in grapevine.Scientific reports · 2018Article
- Small RNA and Transcriptome Sequencing Reveal a Potential miRNA-Mediated Interaction Network That Functions during Somatic Embryogenesis inFrontiers in plant science · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) is a rapid and sensitive method for analyzing microRNA (miRNA) expression. However, accurate qRT-PCR results depend on the selection of reliable reference genes as internal positive controls. To date, few studies have identified reliable reference genes for differential expression analysis of miRNAs among tissues, and among experimental conditions in plants. In this study, three miRNAs and four non-coding small RNAs (ncRNA) were selected as reference candidates, and the stability of their expression was evaluated among different tissues and under different experimental conditions in the tea plant (Camellia sinensis) using the geNorm and NormFinder programs. It was shown that miR159a was the best single reference gene in the bud to the fifth leaf, 5S rRNA was the most suitable gene in different organs, miR6149 was the most stable gene when the leaves were attacked by Ectropis oblique and U4, miR5368n and miR159a were the best genes when the leaves were treated by methyl jasmonate (MeJA), salicylic acid (SA) and abscisic acid (ABA), respectively. Our results provide suitable reference genes for future investigations on miRNA functions in tea plants.
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