ArticleNature biotechnology2016
An artificial niche preserves the quiescence of muscle stem cells and enhances their therapeutic efficacy.
Article in Nature biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
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Who cites it
110 citing papers in PubMed, 197 citations in OpenAlex.
- The hallmarks of skeletal muscle health.Nature metabolism · 2026Review
- Human growth plates house resting zone sub-populations with features of quiescent stem cells.Bone research · 2026Article
- Mechanical Signaling Regulates DNA Methylation to Maintain Muscle Stem Cell Quiescence.bioRxiv : the preprint server for biology · 2026Article
- Mechanobiological landscape of muscle stem cells.Skeletal muscle · 2026Review
- Collagen Scaffold Viscoelasticity Regulates Muscle Cell Phenotype.Advanced healthcare materials · 2026Article
- Biomimetic Scaffolds and Extracellular Matrix-Based Strategies for Myofiber Regeneration in Volumetric Muscle Loss.Drug design, development and therapy · 2026Review
- Innovations in skeletal muscle regeneration: from physiology to bioengineering approaches for repair and restoration.Frontiers in physiology · 2026Review
- Regarding: In Vivo Evaluation of Decellularised Skeletal Muscle Matrices for Skeletal Muscle Repair: A Systematic Review.Journal of cellular and molecular medicine · 2025Article
- In vivo self-renewal and expansion of quiescent stem cells from a non-human primate.Nature communications · 2025Article
- Organoid culture promotes dedifferentiation of mouse myoblasts into stem cells capable of complete muscle regeneration.Nature biotechnology · 2025Article
- Biomaterial-Based Regenerative Strategies for Volumetric Muscle Loss: Challenges and Solutions.Advances in wound care · 2025Review
- An Efficient Method for Enrichment and In Vitro Propagation of Muscle Stem Cells Derived from Black Sea Bream (Acanthopagrus schlegelii) Skeletal Muscle.Marine biotechnology (New York, N.Y.) · 2025Article
- Metabolic regulation in adult and aging skeletal muscle stem cells.Genes & development · 2025Review
- The impact of the limbal niche interactions on the self-renewal capability of limbal epithelial stem cells.Frontiers in cell and developmental biology · 2025Article
- Engineering large-scale hiPSC-derived vessel-integrated muscle-like lattices for enhanced volumetric muscle regeneration.Trends in biotechnology · 2024Article
- Dormancy-inducing 3D engineered matrix uncovers mechanosensitive and drug-protective FHL2-p21 signaling axis.Science advances · 2024Article
- Quiescent cancer cells induced by high-density cultivation reveals cholesterol-mediated survival and lung metastatic traits.British journal of cancer · 2024Article
- Challenges and Considerations of Preclinical Development for iPSC-Based Myogenic Cell Therapy.Cells · 2024Review
- Quantification of local matrix deposition during muscle stem cell activation using engineered hydrogels.bioRxiv : the preprint server for biology · 2024Article
- A Self-Renewing Biomimetic Skeletal Muscle Construct Engineered using Induced Myogenic Progenitor Cells.Advanced functional materials · 2024Article
50 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
A promising therapeutic strategy for diverse genetic disorders involves transplantation of autologous stem cells that have been genetically corrected ex vivo. A major challenge in such approaches is a loss of stem cell potency during culture. Here we describe an artificial niche for maintaining muscle stem cells (MuSCs) in vitro in a potent, quiescent state. Using a machine learning method, we identified a molecular signature of quiescence and used it to screen for factors that could maintain mouse MuSC quiescence, thus defining a quiescence medium (QM). We also engineered muscle fibers that mimic the native myofiber of the MuSC niche. Mouse MuSCs maintained in QM on engineered fibers showed enhanced potential for engraftment, tissue regeneration and self-renewal after transplantation in mice. An artificial niche adapted to human cells similarly extended the quiescence of human MuSCs in vitro and enhanced their potency in vivo. Our approach for maintaining quiescence may be applicable to stem cells isolated from other tissues.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.