ArticleBioMed research international2016
Isolation and Characterization of a Broad Spectrum Bacteriocin from Bacillus amyloliquefaciens RX7.
Article in BioMed research international, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 52 citations in OpenAlex.
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- Probiotics in piglet: from gut health to pathogen defense mechanisms.Frontiers in immunology · 2024Review
- Simple Purification and Antimicrobial Properties of Bacteriocin-like Inhibitory Substance fromFoods (Basel, Switzerland) · 2023Article
- Perspectives in Searching Antimicrobial Peptides (AMPs) Produced by the Microbiota.Microbial ecology · 2023Review
- Characterization of the Novel Leaderless Bacteriocin, Bawcin, fromInternational journal of molecular sciences · 2023Article
- Antimicrobial and immunoregulatory effects of Lactobacillus delbrueckii 45E against genitourinary pathogens.Journal of biomedical science · 2023Article
- Metabolomic profiles of the liquid state fermentation in co-culture ofFrontiers in microbiology · 2023Article
- Bacteriocin Production by Bacillus Species: Isolation, Characterization, and Application.Probiotics and antimicrobial proteins · 2022Review
- Genomic analysis ofMolecular biology research communications · 2022Article
- Purification, HR-LC-ESI-MS-MS Identification, and Peptide Prediction of Bacteriocin-Like Inhibitory Substances Produced byInternational journal of food science · 2022Article
- Partial Purification and Characterization of Bacteriocin-Like Inhibitory Substances Produced byBioMed research international · 2021Article
- Molecular insights into probiotic mechanisms of action employed against intestinal pathogenic bacteria.Gut microbes · 2020Review
- Antimicrobial and mechanism of antagonistic activity ofFood science & nutrition · 2020Article
- Unveiled feather microcosm: feather microbiota of passerine birds is closely associated with host species identity and bacteriocin-producing bacteria.The ISME journal · 2019Article
- Suppression of bacteriocin resistance using live, heterospecific competitors.Evolutionary applications · 2019Article
- Isolation and Characterization of an Anti-listerial Bacteriocin fromKorean journal for food science of animal resources · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We isolated a Bacillus strain, RX7, with inhibitory activity against Listeria monocytogenes from soil and identified it as Bacillus amyloliquefaciens based on 16S rRNA gene sequencing. The inhibitory activity was stable over a wide range of pH and was fully retained after 30 min at 80°C, after which it decreased gradually at higher temperatures. The activity was sensitive to the proteolytic action of α-chymotrypsin, proteinase-K, and trypsin, indicating its proteinaceous nature. This bacteriocin was active against a broad spectrum of bacteria and the fungus Candida albicans. Direct detection of antimicrobial activity on a sodium dodecyl sulfate-polyacrylamide gel suggested an apparent molecular mass of approximately 5 kDa. Ammonium sulfate precipitation and anion-exchange and gel permeation chromatography integrated with reverse phase-high-performance liquid chromatography were used for bacteriocin purification. Automated N-terminal Edman degradation of the purified RX7 bacteriocin recognized the first 15 amino acids as NH2-X-Ala-Trp-Tyr-Asp-Ile-Arg-Lys-Leu-Gly-Asn-Lys-Gly-Ala, where the letter X in the sequence indicates an unknown or nonstandard amino acid. Based on BLAST similarity search and multiple alignment analysis, the obtained partial sequence showed high homology with the two-peptide lantibiotic haloduracin (HalA1) from Bacillus halodurans, although at least two amino acids differed between the sequences. A time-kill study demonstrated a bactericidal mode of action of RX7 bacteriocin.
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