Evidence map›Paper›PMID 27238716›Full record

ArticlePlacenta2016

Differential patterning of genes involved in serotonin metabolism and transport in extra-embryonic tissues of the mouse.

Hsiao-Huei Wu, Sera Choi, Pat Levitt

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Placenta, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
8.5field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 30 citations in OpenAlex.

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  11. The Shaggy Dog Story of Enteric Signaling: Serotonin, a Molecular Megillah.Advances in experimental medicine and biology · 2022
    Article
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  17. p38α MAPK signaling drives pharmacologically reversible brain and gastrointestinal phenotypes in the SERT Ala56 mouse.Proceedings of the National Academy of Sciences of the United States of America · 2018
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  18. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Hsiao-Huei WuDepartment of Pediatrics, Children's Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Sera ChoiDepartment of Pediatrics, Children's Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
Pat LevittDepartment of Pediatrics, Children's Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA; Institute for the Developing Mind, The Saban Research Institute, Children's Hospital Los Angeles, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA. Electronic address: Plevitt@med.usc.edu.
University of Southern California · US

Funding

Stress-Mediated Alterations in Serotonin 2C Receptor Editing and FunctionP50MH096972 · NIMH · VANDERBILT UNIVERSITY · PI BLAKELY, RANDY D. · 2012 to 2016
$10.5M
Project 6 Interactions of Serotonin and Circadian Signaling NetworksP50MH078028 · NIMH · VANDERBILT UNIVERSITY · PI SNODDY, JAY R · 2007 to 2011
$9.8M
NIMH NIH HHS P50 MH078028NIMH NIH HHS P50 MH096972
6 · The paper itself

Abstract

introductionSerotonin (5-HT) is an important neuromodulator, but recently has been shown to be involved in neurodevelopment. Although previous studies have demonstrated that the placenta is a major source of forebrain 5-HT during early forebrain development, the processes of how 5-HT production, metabolism, and transport from placenta to fetus are regulated are unknown. As an initial step in determining the mechanisms involved, we investigated the expression patterns of genes critical for 5-HT system function in mouse extraembryonic tissues.

methodsMid-through late gestation expression of 5-HT system-related enzymes, Tph1, Ddc, Maoa, and 5-HT transporters, Sert/Slc6a4, Oct3/Slc22a3, Vmat2/Slc18a2, and 5-HT in placenta and yolk sac were examined, with cell type-specific resolution, using multiplex fluorescent in situ hybridization to co-localize transcripts and immunocytochemistry to co-localize the corresponding proteins and neurotransmitter.

resultsTph1 and Ddc are found in the syncytiotrophoblast I (SynT-I) and sinusoidal trophoblast giant cells (S-TGC), whereas Maoa is expressed in SynT-I, syncytiotrophoblast II (SynT-II) and S-TGC. Oct3 expression is observed in the SynT-II only, while Vmat2 is mainly expressed in S-TGC. Surprisingly, there were comparatively high expression of Tph1, Ddc, and Maoa in the yolk sac visceral endoderm. DISCUSSION: In addition to trophoblast cells, visceral endoderm cells in the yolk sac may contribute to fetal 5-HT production. The findings raise the possibility of a more complex regulation of 5-HT access to the fetus through the differential roles of trophoblasts that surround maternal and fetal blood space and of yolk sac endoderm prior to normal degeneration.

Indexed as

Gene ExpressionAnimalsFemaleMiceMonoamine OxidaseOrgan SpecificityPlacentaPregnancySerotoninSerotonin Plasma Membrane Transport ProteinsTryptophan HydroxylaseYolk SacMonoamine OxidaseSerotoninSerotonin Plasma Membrane Transport ProteinsTph1 protein, mouseTryptophan HydroxylasePlacentaSerotoninSinusoidal trophoblast giant cellsSyncytiotrophoblastYolk sac

Identifiers

PMID27238716
PMCPMC4886340
OpenAlexW2332661847

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.