ArticleGastroenterology2016
Mouse Label-Retaining Cells Are Molecularly and Functionally Distinct From Reserve Intestinal Stem Cells.
Article in Gastroenterology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
- Building and regenerating intestines by manipulating intestinal stem cells.Nature reviews. Gastroenterology & hepatology · 2026Review
- Metrnl in mitigating radiation enteritisActa pharmaceutica Sinica. B · 2026Article
- Fasting primes small intestinal regeneration after damage via a microbiome-metabolite-chromatin axis.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Single-nucleus multi-omics analysis of mouse small-intestinal Lgr5+ cell populations reveals Foxa3-induced Paneth cell-lineage differentiation.Communications biology · 2026Article
- Dietary Stearic Acid Accelerates Intestinal Tumorigenesis via Fatty Acid-binding Protein 5 Without Promoting Obesity.Cellular and molecular gastroenterology and hepatology · 2026Article
- Dual states of murine Bmi1-expressing intestinal stem cells drive epithelial development utilizing non-canonical Wnt signaling.Developmental cell · 2025Article
- Phosphatidylinositol 3-Kinase Signaling Enhances Intestinal Crypt Epithelial Cell Recovery after Radiation.The American journal of pathology · 2025Article
- A Lgr5-independent developmental lineage is involved in mouse intestinal regeneration.Development (Cambridge, England) · 2025Article
- Dietary and metabolic effects on intestinal stem cells in health and disease.Nature reviews. Gastroenterology & hepatology · 2025Review
- Current status and new directions for hepatocellular carcinoma diagnosis.Liver research (Beijing, China) · 2024Review
- Toxicity of nuclear-localized GFP in reporter mice.Scientific reports · 2024Article
- Navigating the Landscape of Intestinal Regeneration: A Spotlight on Quiescence Regulation and Fetal Reprogramming.International journal of stem cells · 2024Review
- S100A6 Regulates nucleus pulposus cell apoptosis via Wnt/β-catenin signaling pathway: an in vitro and in vivo study.Molecular medicine (Cambridge, Mass.) · 2024Article
- Article
- Advanced Progression for the Heterogeneity and Homeostasis of Intestinal Stem Cells.Stem cell reviews and reports · 2023Review
- S100A6: molecular function and biomarker role.Biomarker research · 2023Review
- Spatially resolved expression landscape and gene-regulatory network of human gastric corpus epithelium.Protein & cell · 2023Article
- S100A6 as a Constituent and Potential Marker of Adult and Cancer Stem Cells.Stem cell reviews and reports · 2022Review
- Autophagic state prospectively identifies facultative stem cells in the intestinal epithelium.EMBO reports · 2022Article
- Intestinal cellular heterogeneity and disease development revealed by single-cell technology.Cell regeneration (London, England) · 2022Review
4 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors.
Funding
Abstract
BACKGROUND &
aimsIntestinal homeostasis and regeneration after injury are controlled by 2 different types of cells: slow cycling, injury-resistant reserve intestinal stem cells (ISCs) and actively proliferative ISCs. Putative reserve ISCs have been identified using a variety of methods, including CreER insertions at Hopx or Bmi1 loci in mice and DNA label retention. Label-retaining cells (LRCs) include dormant stem cells in several tissues; in the intestine, LRCs appear to share some properties with reserve ISCs, which can be marked by reporter alleles. We investigated the relationships between these populations.
methodsStudies were performed in Lgr5-EGFP-IRESCreERT2, Bmi1-CreERT2, Hopx-CreERT2, and TRE-H2BGFP::Hopx-CreERT2::lox-stop-lox-tdTomato mice. Intestinal epithelial cell populations were purified; we compared reporter allele-marked reserve ISCs and several LRC populations (marked by H2B-GFP retention) using histologic flow cytometry and functional and single-cell gene expression assays.
resultsLRCs were dynamic and their cellular composition changed with time. Short-term LRCs had properties of secretory progenitor cells undergoing commitment to the Paneth or enteroendocrine lineages, while retaining some stem cell activity. Long-term LRCs lost stem cell activity and were a homogenous population of terminally differentiated Paneth cells. Reserve ISCs marked with HopxCreER were primarily quiescent (in G0), with inactive Wnt signaling and robust stem cell activity. In contrast, most LRCs were in G1 arrest and expressed genes that are regulated by the Wnt pathway or are in the secretory lineage.
conclusionsLRCs are molecularly and functionally distinct from reporter-marked reserve ISCs. This information provides an important basis for future studies of relationships among ISC populations.
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