ArticleBiologicals : journal of the International Association of Biological Standardization2016
Rhabdovirus-like endogenous viral elements in the genome of Spodoptera frugiperda insect cells are actively transcribed: Implications for adventitious virus detection.
Article in Biologicals : journal of the International Association of Biological Standardization, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 43 citations in OpenAlex.
- Endogenous RNA virus-derived elements exhibit functional divergence in modulating development and virus susceptibility inVirulence · 2026Article
- Tracing ancient viral footprints: a comprehensive study of endogenous viral elements in Bombus species.Mobile DNA · 2026Article
- Mosquito transgenerational antiviral immunity is mediated by vertical transfer of virus DNA sequences and RNAi.iScience · 2024Article
- A tale of caution: How endogenous viral elements affect virus discovery in transcriptomic data.Virus evolution · 2024Article
- Evaluation of recombinant baculovirus clearance during rAAV production in Sf9 cells using a newly developed fluorescent-TCIDFrontiers in medicine · 2024Article
- Host ranges of Sf-rhabdoviruses harbored by lepidopteran insects and insect cell lines.Virology · 2023Article
- Genomic Analysis of Amphioxus Reveals a Wide Range of Fragments Homologous to Viral Sequences.Viruses · 2023Article
- Review
- Insights into the quality of recombinant proteins produced by two different Bombyx mori expression systems.Scientific reports · 2022Article
- Article
- Novel RNA Viruses from the Transcriptome of Pheromone Glands in the Pink Bollworm Moth,Insects · 2021Article
- A new nodavirus-negative Trichoplusia ni cell line for baculovirus-mediated protein production.Biotechnology and bioengineering · 2020Article
- Human Tibroviruses: Commensals or Lethal Pathogens?Viruses · 2020Review
- Next-generation cell lines established from the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae).In vitro cellular & developmental biology. Animal · 2019Article
- Host Range and Population Survey of Spodoptera frugiperda Rhabdovirus.Journal of virology · 2019Article
- The complete genome sequence of CrRV-Ch01, a new member of the family Rhabdoviridae in the parasitic copepod Caligus rogercresseyi present on farmed Atlantic salmon (Salmo salar) in Chile.Archives of virology · 2018Article
- Adventitious viruses in insect cell lines used for recombinant protein expression.Protein expression and purification · 2018Review
- A new approach for detecting adventitious viruses shows Sf-rhabdovirus-negative Sf-RVN cells are suitable for safe biologicals production.BMC biotechnology · 2018Article
- Potential Role of Accessory Domains in Polyproteins Encoded by Retrotransposons in Anti-viral Defense of Host Cells.Frontiers in microbiology · 2018Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Spodoptera frugiperda (Sf) cell lines are used to produce several biologicals for human and veterinary use. Recently, it was discovered that all tested Sf cell lines are persistently infected with Sf-rhabdovirus, a novel rhabdovirus. As part of an effort to search for other adventitious viruses, we searched the Sf cell genome and transcriptome for sequences related to Sf-rhabdovirus. To our surprise, we found intact Sf-rhabdovirus N- and P-like ORFs, and partial Sf-rhabdovirus G- and L-like ORFs. The transcribed and genomic sequences matched, indicating the transcripts were derived from the genomic sequences. These appear to be endogenous viral elements (EVEs), which result from the integration of partial viral genetic material into the host cell genome. It is theoretically impossible for the Sf-rhabdovirus-like EVEs to produce infectious virus particles as 1) they are disseminated across 4 genomic loci, 2) the G and L ORFs are incomplete, and 3) the M ORF is missing. Our finding of transcribed virus-like sequences in Sf cells underscores that MPS-based searches for adventitious viruses in cell substrates used to manufacture biologics should take into account both genomic and transcribed sequences to facilitate the identification of transcribed EVE's, and to avoid false positive detection of replication-competent adventitious viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.