Evidence map›Paper›PMID 27235324›Full record

ArticleToxicologic pathology2016

Tracking Epidermal Nerve Fiber Changes in Asian Macaques: Tools and Techniques for Quantitative Assessment.

Lisa M Mangus, Jamie L Dorsey, Rachel L Weinberg, Gigi J Ebenezer, Peter Hauer, Victoria A Laast, Joseph L Mankowski

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxicologic pathology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.3field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 15 citations in OpenAlex.

  1. Review
  2. A Switch from Glial to Neuronal Gene Expression Alterations in the Spinal Cord of SIV-infected Macaques on Antiretroviral Therapy.Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Lisa M MangusDepartment of Molecular and Comparative Pathobiology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Jamie L DorseyDepartment of Molecular and Comparative Pathobiology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Rachel L WeinbergDepartment of Molecular and Comparative Pathobiology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Gigi J EbenezerDepartment of Neurology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA.
Peter HauerDepartment of Neurology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA Current Affiliation: Department of Neurology, School of Medicine, University of Utah, Salt Lake City, Utah, USA.
Victoria A LaastDepartment of Molecular and Comparative Pathobiology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA Current Affiliation: Covance Pharmaceutical Research and Development (Shanghai) Co. Ltd., Shanghai, China.
Joseph L MankowskiDepartment of Molecular and Comparative Pathobiology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA Department of Neurology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA Department of Pathology, School of Medicine, Johns Hopkins University, Baltimore, Maryland, USA jmankows@jhmi.edu.
Johns Hopkins University · USJohns Hopkins Medicine · USUniversity of Utah · US

Funding

Project 4: Intracellular mechanism of virus persistence in astrocytes/macrophagesP01MH070306 · NIMH · JOHNS HOPKINS UNIVERSITY · PI CLEMENTS, JANICE E · 2004 to 2015
$16.0M
Training Veterinarians for Careers in Biomedical ResearchT32OD011089 · OD · JOHNS HOPKINS UNIVERSITY · PI JOSEPH L MANKOWSKI · 2012 to 2026
$6.7M
Development of an SPF Macaca nemestrina Breeding ColonyP40OD013117 · OD · JOHNS HOPKINS UNIVERSITY · PI ADAMS, ROBERT J. · 2012 to 2016
$4.9M
Novel SIV Model of HANDR01NS077869 · NINDS · JOHNS HOPKINS UNIVERSITY · PI CLEMENTS, JANICE E, MANKOWSKI, JOSEPH L · 2012 to 2016
$3.8M
Eradicating Latent SIV from the CNS by CCR5 InhibitionR01NS089482 · NINDS · JOHNS HOPKINS UNIVERSITY · PI MANKOWSKI, JOSEPH L · 2014 to 2018
$3.8M
Mechanisms of HIV-Induced PNS Disease: The SIV Macaque ModelR01NS055651 · NINDS · JOHNS HOPKINS UNIVERSITY · PI MANKOWSKI, JOSEPH L · 2006 to 2009
$2.5M
NIH HHS P40 OD013117NIH HHS T32 OD011089NIMH NIH HHS P01 MH070306NINDS NIH HHS R01 NS055651NINDS NIH HHS R01 NS077869NINDS NIH HHS R01 NS089482
6 · The paper itself

Abstract

Quantitative assessment of epidermal nerve fibers (ENFs) has become a widely used clinical tool for the diagnosis of small fiber neuropathies such as diabetic neuropathy and human immunodeficiency virus-associated sensory neuropathy (HIV-SN). To model and investigate the pathogenesis of HIV-SN using simian immunodeficiency virus (SIV)-infected Asian macaques, we adapted the skin biopsy and immunostaining techniques currently employed in human patients and then developed two unbiased image analysis techniques for quantifying ENF in macaque footpad skin. This report provides detailed descriptions of these tools and techniques for ENF assessment in macaques and outlines important experimental considerations that we have identified in the course of our long-term studies. Although initially developed for studies of HIV-SN in the SIV-infected macaque model, these methods could be readily translated to a range of studies involving peripheral nerve degeneration and neurotoxicity in nonhuman primates as well as preclinical investigations of agents aimed at neuroprotection and regeneration.

Indexed as

AnimalsBiopsyImage Processing, Computer-AssistedMacacaNerve DegenerationNerve FibersPeripheral Nervous System DiseasesSimian Acquired Immunodeficiency SyndromeSkinepidermal nerve fibersimage analysisnervous systemnonhuman primateperipheral neuropathyPGP9.5stereology

Identifiers

PMID27235324
PMCPMC4965306
OpenAlexW2397014632

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.