ArticlePlasmid2016
New high-cloning-efficiency vectors for complementation studies and recombinant protein overproduction in Escherichia coli and Salmonella enterica.
Article in Plasmid, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
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- Protein STM3547 From Salmonella typhimurium Is a Phosphofructose Kinase B-Type Enzyme With Ribose Kinase Activity.Molecular microbiology · 2026Article
- In Salmonella Typhimurium, YiiD Modulates cAMP Levels in Lag Phase During Growth on Succinate.Molecular microbiology · 2026Article
- The XRE family protein DbuR is a transcriptional repressor of theApplied and environmental microbiology · 2025Article
- Biochemical and structural characterization of a reactive intermediate deaminase A homolog from Streptococcus sanguinis.Scientific reports · 2025Article
- InPathogens (Basel, Switzerland) · 2025Article
- PlsX and PlsY: Additional roles beyond glycerophospholipid synthesis in Gram-negative bacteria.mBio · 2024Article
- A novel vaccine strategy using quick and easy conversion of bacterial pathogens to unnatural amino acid-auxotrophic suicide derivatives.Microbiology spectrum · 2024Article
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- In Salmonella enterica, the pathogenicity island 2 (SPI-2) regulator PagR regulates its own expression and the expression of a five-gene operon that encodes transketolase C.Molecular microbiology · 2023Article
- The coenzyme BMicrobial cell (Graz, Austria) · 2023Article
- DadY (PA5303) is required for fitness ofMicrobial cell (Graz, Austria) · 2022Article
- Localization and interaction studies of the Salmonella enterica ethanolamine ammonia-lyase (EutBC), its reactivase (EutA), and the EutT corrinoid adenosyltransferase.Molecular microbiology · 2022Article
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- Genetic Analysis Using Vitamin BJournal of bacteriology · 2022Article
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- Loss of YggS (COG0325) impacts aspartate metabolism in Salmonella enterica.Molecular microbiology · 2021Article
- Sirtuin-Dependent Reversible Lysine Acetylation Controls the Activity of Acetyl Coenzyme A Synthetase in Campylobacter jejuni.Journal of bacteriology · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Galloway et al. recently described a method to alter vectors to include Type IIS restriction enzymes for high efficiency cloning. Utilizing this method, the multiple cloning sites of complementation and overexpression vectors commonly used in our laboratory were altered to contain recognition sequences of the Type IIS restriction enzyme, BspQI. Use of this enzyme increased the rate of cloning success to >97% efficiency. L(+)-Arabinose-inducible complementation vectors and overexpression vectors encoding N-terminal recombinant tobacco etch virus protease (rTEV)-cleavable H6-tags were altered to contain BspQI sites that allowed for cloning into all vectors using identical primer overhangs. Additionally, a vector used for directing the synthesis of proteins with a C-terminal, rTEV-cleavable H6-tag was engineered to contain BspQI sites, albeit with different overhangs from that of the previously mentioned vectors. Here we apply a method used to engineer cloning vectors to contain BspQI sites and the use of each vector in either in vivo complementation studies or in vitro protein purifications.
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