Evidence map›Paper›PMID 27234384›Full record

ArticleTranslational respiratory medicine2013

A linear polyethylenimine mediated siRNA-based therapy targeting human epidermal growth factor receptor in SPC-A1 xenograft mice.

Pinghai Zhang, Nuo Xu, Lei Zhou, Xin Xu, Yuehong Wang, Ka Li, Zhaochong Zeng, Xiangdong Wang, Xin Zhang, Chunxue Bai

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational respiratory medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Nanoparticles and cytokine response.Frontiers in bioengineering and biotechnology · 2023
    Review
  2. Nanoparticulate RNA delivery systems in cancer.Cancer reports (Hoboken, N.J.) · 2020
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Pinghai ZhangDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
Nuo XuDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
Lei ZhouDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
Xin XuDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
Yuehong WangDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
Ka LiDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
Zhaochong ZengDepartment of Radiotherapy, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
Xiangdong WangDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
Xin ZhangDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 20032, China.
Chunxue BaiDepartment of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, 20032, China. bai.chunxue@zs-hospital.sh.cn.
Sun Yat-sen University · CNThe First Affiliated Hospital, Sun Yat-sen University · CNFudan University · CNZhongshan Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundLinear polyethylenimine (LPEI) is considered as a desirable gene in vivo delivery system, but whether it could deliver the specific siRNA targeted EGFR to the tumor site to inhibit the growth of NSCLC xenograft in nude mice still needs to be examined.

methodsIn this study, LPEI/siRNA was made into a complex and SPC-A1-xenografted mice model was established. Then, stable LPEI/siRNA-EGFR complexes were intraperitoneally administrated. Afterwards, tumor growth was measured every 3 days. At the end of the experiment, tumor volume was calculated, and tumors were weighed, and examined for EGFR expression, proliferation, and apoptosis evaluations. By using blood samples, toxicity tests including aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea and creatinine (Cr) were measured for liver and renal function evaluation. Serum concentrations of TNF-α and IFN-γ were also examined.

resultsOur results demonstrated that LPEI/siRNA-EGFR complexes could downregulate EGFR expression in SPC-A1 xenografted tumor upon single i.p. injection. LPEI/siRNA-EGFR complexes inhibited tumor growth and did not induce organ toxicity in SPC-A1-xenografted mice. At the end of the experiment no significant IFN-α increase was detected in LPEI/siRNA complexes or glucose-treated groups.

conclusionsThe novel modality of siRNA-based therapy targeting EGFR may be of great potential in NSCLC treatment.

Indexed as

Epidermal growth factor receptorIntraperitoneal injectionLinear-polyethylenimineNon small cell lung cancerRNA interferenceSmall interfering RNA

Identifiers

PMID27234384
PMCPMC6733432
OpenAlexW2132685390

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.