ReviewAnaerobe2016
A roadmap for gene system development in Clostridium.
Review in Anaerobe, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed, 107 citations in OpenAlex.
- The association between polyunsaturated fatty acids in breast milk and infant eczema and its relationship with infant gut microbiota.Asia Pacific journal of clinical nutrition · 2026Article
- SMC-like Wadjet system prevents plasmid transfer into Clostridium cellulovorans.Applied microbiology and biotechnology · 2025Article
- Deletion of atypical type II restriction genes in Clostridium cellulovorans using a Cas9-based gene editing system.Applied microbiology and biotechnology · 2025Article
- Recent progress in engineeringSynthetic and systems biotechnology · 2024Review
- Genetic tools for the electrotrophApplied and environmental microbiology · 2024Article
- Development of a genetic engineering toolbox for syngas-utilizing acetogen Clostridium sp. AWRP.Microbial cell factories · 2024Article
- The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024Review
- The Restriction-Modification Systems ofMicroorganisms · 2023Article
- Article
- Genetic Engineering of Resident Bacteria in the Gut Microbiome.Journal of bacteriology · 2023Review
- Exploitation of a Type 1 Toxin-Antitoxin System as an Inducible Counter-Selective Marker for Genome Editing in the AcetogenMicroorganisms · 2023Article
- Developing a genetic engineering method for Acetobacterium wieringae to expand one-carbon valorization pathways.Biotechnology for biofuels and bioproducts · 2023Article
- Base editing enables duplex point mutagenesis inFrontiers in bioengineering and biotechnology · 2023Article
- Chromosomal engineering of inducible isopropanol- butanol-ethanol production inFrontiers in bioengineering and biotechnology · 2023Article
- Refined control of CRISPR-Cas9 gene editing inFrontiers in immunology · 2023Article
- Diversity, Dynamics and Therapeutic Application ofViruses · 2022Review
- Required Gene Set for Autotrophic Growth ofApplied and environmental microbiology · 2022Article
- Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum.Scientific reports · 2022Article
- Article
- Reversible Hydrogenase Activity Confers Flexibility to Balance Intracellular Redox inFrontiers in microbiology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Clostridium species are both heroes and villains. Some cause serious human and animal diseases, those present in the gut microbiota generally contribute to health and wellbeing, while others represent useful industrial chassis for the production of chemicals and fuels. To understand, counter or exploit, there is a fundamental requirement for effective systems that may be used for directed or random genome modifications. We have formulated a simple roadmap whereby the necessary gene systems maybe developed and deployed. At its heart is the use of 'pseudo-suicide' vectors and the creation of a pyrE mutant (a uracil auxotroph), initially aided by ClosTron technology, but ultimately made using a special form of allelic exchange termed ACE (Allele-Coupled Exchange). All mutants, regardless of the mutagen employed, are made in this host. This is because through the use of ACE vectors, mutants can be rapidly complemented concomitant with correction of the pyrE allele and restoration of uracil prototrophy. This avoids the phenotypic effects frequently observed with high copy number plasmids and dispenses with the need to add antibiotic to ensure plasmid retention. Once available, the pyrE host may be used to stably insert all manner of application specific modules. Examples include, a sigma factor to allow deployment of a mariner transposon, hydrolases involved in biomass deconstruction and therapeutic genes in cancer delivery vehicles. To date, provided DNA transfer is obtained, we have not encountered any clostridial species where this technology cannot be applied. These include, Clostridium difficile, Clostridium acetobutylicum, Clostridium beijerinckii, Clostridium botulinum, Clostridium perfringens, Clostridium sporogenes, Clostridium pasteurianum, Clostridium ljungdahlii, Clostridium autoethanogenum and even Geobacillus thermoglucosidasius.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.