ArticleMicrobial cell factories2016
Engineering Escherichia coli for production of 4-hydroxymandelic acid using glucose-xylose mixture.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 49 citations in OpenAlex.
- Engineering mixed sugar metabolic channels inSynthetic and systems biotechnology · 2026Article
- Metabolic engineering of Escherichia coli for resveratrol production using food-grade D-xylose as a carbon source.Journal of industrial microbiology & biotechnology · 2026Article
- EngineeringBiodesign research · 2025Article
- Production of aromatic amino acids and their derivatives by Escherichia coli and Corynebacterium glutamicum.World journal of microbiology & biotechnology · 2025Review
- Expanding the application of tyrosine: engineering microbes for the production of tyrosine and its derivatives.Frontiers in bioengineering and biotechnology · 2025Review
- Co-production of biofuel, bioplastics and biochemicals during extended fermentation of Halomonas bluephagenesis.Microbial biotechnology · 2023Article
- Adaptation on xylose improves glucose-xylose co-utilization and ethanol production in a carbon catabolite repression (CCR) compromised ethanologenic strain.Microbial cell factories · 2022Article
- Directed evolution of the PobR allosteric transcription factor to generate a biosensor for 4-hydroxymandelic acid.World journal of microbiology & biotechnology · 2022Article
- Rewiring the microbial metabolic network for efficient utilization of mixed carbon sources.Journal of industrial microbiology & biotechnology · 2021Article
- Improving CoQMicrobial cell factories · 2021Article
- Chromosome Engineering To Generate Plasmid-Free Phenylalanine- and Tyrosine-OverproducingApplied and environmental microbiology · 2020Article
- Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers.Metabolic engineering · 2020Article
- Recent Advances in Metabolically Engineered Microorganisms for the Production of Aromatic Chemicals Derived From Aromatic Amino Acids.Frontiers in bioengineering and biotechnology · 2020Review
- Metabolic engineering of microorganisms for production of aromatic compounds.Microbial cell factories · 2019Review
- Metabolic engineeringBiotechnology for biofuels · 2019Article
- Metabolic Engineering ofFrontiers in bioengineering and biotechnology · 2018Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
background4-Hydroxymandelic acid (4-HMA) is a valuable aromatic fine chemical and widely used for production of pharmaceuticals and food additives. 4-HMA is conventionally synthesized by chemical condensation of glyoxylic acid with excessive phenol, and the process is environmentally unfriendly. Microbial cell factory would be an attractive approach for 4-HMA production from renewable and sustainable resources.
resultsIn this study, a biosynthetic pathway for 4-HMA production was constructed by heterologously expressing the fully synthetic 4-hydroxymandelic acid synthase (shmaS) in our L-tyrosine-overproducing Escherichia coli BKT5. The expression level of shmaS was optimized to improve 4-HMA production by fine tuning of four promoters of different strength combined with three plasmids of different copy number. Furthermore, two genes aspC and tyrB in the competitive pathway were deleted to block the formation of byproduct to enhance 4-HMA biosynthesis. The final engineered E. coli strain HMA15 utilized glucose and xylose simultaneously and produced 15.8 g/L of 4-HMA by fed-batch fermentation in 60 h.
conclusionsMetabolically engineered E. coli strain for 4-HMA production was designed and constructed, and efficiently co-fermented glucose and xylose, the major components in the hydrolysate mixture of agricultural biomass. Our research provided a promising biomanufacturing route to produce 4-HMA from lignocellulosic biomass.
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