ArticleReproductive biology and endocrinology : RB&E2016
Effect of nerve growth factor on sperm quality in asthenozoosprmic men during cryopreservation.
Article in Reproductive biology and endocrinology : RB&E, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Effects of Platelet-Rich Plasma Dose and Application Strategy on Post-Thaw Spermatological Parameters in Goat Semen.Veterinary sciences · 2026Article
- L-Proline supplementation preserves sperm function and chromatin integrity in asthenozoospermic patients during cryopreservation.Basic and clinical andrology · 2025Article
- A comprehensive meta-analysis on the efficacy of growth factor enriched cryo-agents in enhancing post-thaw quality of mammalian semen.Animal bioscience · 2025Article
- Effect of Recombinant NGF Encapsulated in Chitosan on Rabbit Sperm Traits and Main Metabolic Pathways.Biology · 2025Article
- Safety of Plasma Rich in Growth Factors (PRGF) as additive to healthy human sperm samples: a pilot study.JBRA assisted reproduction · 2024Article
- Effect of Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cells on Human Sperm Quality During Cryopreservation.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- NGF, EPO, and IGF-1 in the Male Reproductive System.Journal of clinical medicine · 2024Review
- A Possible Role for Nerve Growth Factor and Its Receptors in Human Sperm Pathology.Biomedicines · 2023Article
- The role of growth factors in human sperm parameters: A review of in vitro studies.International journal of reproductive biomedicine · 2022Review
- Histopathologic evaluation of the inflammatory factors and stromal cells in the endometriosis lesions: A case-control study.International journal of reproductive biomedicine · 2022Article
- Expression of Nerve Growth Factor and Its Receptor TrkA in the Reproductive System of Adult Zebrafish.Veterinary sciences · 2022Article
- Serum Nerve Growth Factor Levels as a Predictor of Bull Candidate Semen Quality of Madura Cattle.Veterinary medicine international · 2022Article
- IGF-I and NGFβ enhance in vitro progressive motility and vitality of human spermatozoa.Reproductive medicine and biology · 2021Article
- Nerve Growth Factor (NGF) and Animal Reproduction.Advances in experimental medicine and biology · 2021Review
- Curcumin improves asthenozoospermia by inhibiting reactive oxygen species reproduction through nuclear factor erythroid 2-related factor 2 activation.Andrologia · 2020Article
- Effects of NGF Addition on Llama (Frontiers in veterinary science · 2020Article
- Improvement of Human Sperm Vacuolization and DNA Fragmentation Co-Cultured with Adipose-Derived Mesenchymal Stem Cell Secretome:International journal of stem cells · 2019Article
- In vitro effect of nerve growth factor on the main traits of rabbit sperm.Reproductive biology and endocrinology : RB&E · 2019Article
- The roles of reactive oxygen species and antioxidants in cryopreservation.Bioscience reports · 2019Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlthough routinely used in assisted reproductive technology, human sperm cryopreservation is not an entirely successful procedure. This study determined the effect of nerve growth factor (NGF) supplementation of cryopreservation medium on post-thaw viability, motility, intracellular nitric oxide (NO) concentration, and DNA fragmentation of human spermatozoa in asthenozoospermic men.
methodsSemen samples were collected from 25 asthenozoosprmic men and divided into the following groups (n = 5/group): fresh semen (control); frozen-thawed semen without treatment; frozen-thawed semen with NGF treatment (0.5, 1, and 5 ng/ml). Prior to dividing the asthenozoospermic samples, 200 μl of each sample was collected for NGF content assessment by ELISA and then compared with normozoospermic semen samples (25 normozoospermic men). Sperm motility and viability were assessed according to WHO criteria. Furthermore, intracellular nitric oxide and DNA fragmentation were evaluated by Flow Cytometry.
resultsNGF content was significantly higher in normozoospermic compared with asthenozoospermic men. Cryopreservation of asthenozoospermic semen samples significantly decreased sperm viability and motility, and increased intracellular nitric oxide concentration and DNA damage (p < 0.01). In asthenozoospermic frozen-thawed samples treated with 0.5 ng/ml exogenous NGF, we observed a significantly increased viability, motility, and decreased DNA fragmentation (p < 0.05), but intracellular nitric oxide concentration was not reduced. The other high doses (1 and 5 ng/ml) had no significant effect on the variables.
conclusionSupplementation with exogenous NGF could have partial and limited protective effect during cryopreservation of human spermatozoa but further research is needed to evaluate the possible clinical applications.
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