ArticleThe Journal of general virology2016
Enhanced prion detection in biological samples by magnetic particle extraction and real-time quaking-induced conversion.
Article in The Journal of general virology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
What it found
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Who cites it
42 citing papers in PubMed, 55 citations in OpenAlex.
- From a prion seed to a tree of discoveries: Byron Caughey's RT-QuIC legacy in protein misfolding research.Acta neuropathologica · 2026Review
- Article
- Prion shedding is reduced by chronic wasting disease vaccination.PLoS pathogens · 2026Article
- Practical Real-Time Quaking-Induced Conversion for Detecting Classical Bovine Spongiform Encephalopathy and Classical and Atypical Scrapie Prions.Pathogens (Basel, Switzerland) · 2026Article
- Comparison of acetone and sodium phosphotungstic acid precipitation for sample enrichment prior to RT-QuIC for the detection of prion disease.BMC research notes · 2026Article
- Quantification and diagnostic relevance of blood and heme-mediated inhibition of prion detection by RT-QuIC.Journal of clinical microbiology · 2026Article
- Prion shedding is reduced by chronic wasting disease vaccination.bioRxiv : the preprint server for biology · 2025Article
- Proof-of-concept for an alternative prion detection method utilizing nanoparticle-enhanced, aptamer-template loop-mediated isothermal amplification.Scientific reports · 2025Article
- Vertical transmission of chronic wasting disease in free-ranging white-tailed deer populations.Scientific reports · 2025Article
- Tracking Prions by RT-QuIC: An Update.Sub-cellular biochemistry · 2025Review
- Evaluating the Diagnostic Efficacy of Using Pooled Samples for Chronic Wasting Disease Testing and Surveillance.Pathogens (Basel, Switzerland) · 2024Article
- Comparative study of immunoassays, a microelectromechanical systems-based biosensor, and RT-QuIC for the diagnosis of chronic wasting disease in white-tailed deer.BMC veterinary research · 2024Article
- Temporal Characterization of Prion Shedding in Secreta of White-Tailed Deer in Longitudinal Study of Chronic Wasting Disease, United States.Emerging infectious diseases · 2024Article
- Prion Seeding Activity in Plant Tissues Detected by RT-QuIC.Pathogens (Basel, Switzerland) · 2024Article
- RT-QuIC detection of chronic wasting disease prion in platelet samples of white-tailed deer.BMC veterinary research · 2024Article
- Chronic Wasting Disease: State of the Science.Pathogens (Basel, Switzerland) · 2024Review
- Detection of prions in the urine of patients affected by sporadic Creutzfeldt-Jakob disease.Annals of clinical and translational neurology · 2023Article
- Dynamics of CWD prion detection in feces and blood from naturally infected white-tailed deer.Scientific reports · 2023Article
- Large animal models for chronic wasting disease.Cell and tissue research · 2023Review
- Development of a sensitive real-time quaking-induced conversion (RT-QuIC) assay for application in prion-infected blood.PloS one · 2023Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Prions have been demonstrated in body fluids and excreta using bioassay, but at levels too low for detection by conventional direct-detection assays. More rapid and sensitive detection of prions in these clinically accessible specimens would be valuable for diagnosis and investigations of transmission, environmental impact, and interventions. In addition to very low concentrations of prions, in vitro amplification assays are challenged by the presence of inhibitors in these complex sources. Here, we leverage the prion attribute of avid metal binding with the versatile power of real-time quaking-induced conversion (RT-QuIC) to enhance and simplify detection of chronic wasting-disease prions in biological samples. Iron oxide particle binding and magnetic extraction combined with RT-QuIC permitted rapid analysis of the low concentrations of prions in saliva, urine, faeces, and cerebrospinal fluid. These methods are pertinent to ante-mortem detection, monitoring, and surveillance, and could conceivably be applicable to other protein-misfolding disorders.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.