ArticleJournal of assisted reproduction and genetics2016
Closed vitrification of human oocytes and blastocysts: outcomes from a series of clinical cases.
Article in Journal of assisted reproduction and genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed, 1 synthesis or guideline pooled it, 26 citations in OpenAlex.
- Open versus closed vitrification system of human oocytes and embryos: a systematic review and meta-analysis of embryologic and clinical outcomes.Reproductive biology and endocrinology : RB&E · 2018Pooled it
- Vitrification altered αV and β3 integrin expression in mouse oocytes in a maturation stage-dependent manner.Molecular biology reports · 2026Article
- Validation and clinical study of single-step vitrification combined with single-step warming of human blastocysts.Journal of assisted reproduction and genetics · 2026Article
- The impact of completely shrunken blastocysts on clinical outcomes in vitrified-warmed single blastocyst transfer cycles.Scientific reports · 2025Article
- Comparative Assessment of Survival and Clinical Outcome Between Two Commercial Vitrification Kits with Different Warming Protocols After Blastocyst Culture: Potential Perspectives Toward Simplified Warming Procedures.Reproductive sciences (Thousand Oaks, Calif.) · 2023Article
- Comparison of the effect of two commercialized vitrification carriers on pregnancy outcomes in freeze-thaw cycles.The Journal of international medical research · 2023Article
- Effect of mouse ovarian tissue cryopreservation by vitrification with Rapid-i closed system.Journal of assisted reproduction and genetics · 2018Article
- Risk of Contamination of Gametes and Embryos during Cryopreservation and Measures to Prevent Cross-Contamination.BioMed research international · 2017Review
- The cytotoxic effects of dimethyl sulfoxide in mouse preimplantation embryos: a mechanistic study.Theranostics · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeHigh survival rates and clinical outcomes similar to those from fresh oocytes and blastocysts have been observed with open oocyte vitrification systems. It has been suggested that the extremely fast cooling rates that are only achieved with open systems are necessary for human oocyte and blastocyst vitrification. However, there is a potential risk of introducing contamination with open systems. The aim of this study was to assess whether similar survival and subsequent implantation rates could be achieved using a closed vitrification system for human oocytes and blastocysts.
methodsInitially, donated immature oocytes that were matured in vitro were vitrified using the cryoprotectants ethylene glycol (EG) + dimethyl sulphoxide (DMSO) + sucrose and either a closed system (Rapid-i®) or an open system (Cryolock). The closed system was subsequently introduced clinically for mature oocyte cryopreservation cases and blastocyst vitrification.
resultsUsing in vitro matured oocytes, a similar survival was achieved with the open system of 92.4 % (73/79) and with the closed system of 89.7 % (35/39). For clinical oocyte closed vitrification, high survival rate of 90.5 % (374/413) and an implantation rate of 32.7 % (18/55) from the transfer of day 2 embryos was achieved, which is similar to fresh day 2 embryo transfers. Blastocysts have also been successfully cryopreserved using the Rapid-i closed vitrification system with 94 % of blastocysts having an estimated ≥75 % of cells intact and a similar implantation rate (31.5 %) to fresh single blastocyst transfers.
conclusionClosed vitrification can achieve high survival and similar implantation rates to fresh for both oocytes and blastocysts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.