ArticlePLoS genetics2016
The Gene Expression Program for the Formation of Wing Cuticle in Drosophila.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Heat-mediated manipulation of gene expression by IR-LEGO in the developing genitalia in Drosophila.G3 (Bethesda, Md.) · 2026Article
- Mechanical control of the insect extracellular matrix nanostructure.Science advances · 2026Article
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- Entangled and non-modular enhancer sequences producing independent spatial activities.Science advances · 2024Article
- Specialized structure and function of the apical extracellular matrix at sense organs.Cells & development · 2024Review
- Apical cell expansion maintained by Dusky-like establishes a scaffold for corneal lens morphogenesis.Science advances · 2024Article
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- The SWI/SNF nucleosome remodeler constrains enhancer activity during Drosophila wing development.Genetics · 2024Article
- Cellular heterogeneity of the developing worker honey bee (Apis mellifera) pupa: a single cell transcriptomics analysis.G3 (Bethesda, Md.) · 2023Article
- Article
- A Blueprint of Microstructures and Stage-Specific Transcriptome Dynamics of Cuticle Formation inInternational journal of molecular sciences · 2022Article
- The wing imaginal disc.Genetics · 2022Article
- Cell cycle expression of polarity genes features Rb targeting of Vang.Cells & development · 2022Article
- Silencing ofFrontiers in physiology · 2022Article
- Fluorescent Microscopy-Based Detection of Chitin in IntactFrontiers in physiology · 2022Article
- Lint, a transmembrane serine protease, regulates growth and metabolism in Drosophila.Genetics · 2021Article
- Mud Loss Restricts Yki-Dependent Hyperplasia inJournal of developmental biology · 2020Article
- Insect Mouthpart Transcriptome Unveils Extension of Cuticular Protein Repertoire and Complex Organization.iScience · 2020Article
- The tanning hormone, bursicon, does not act directly on the epidermis to tan the Drosophila exoskeleton.BMC biology · 2020Article
- Insect Cuticular Chitin Contributes to Form and Function.Current pharmaceutical design · 2020Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The cuticular exoskeleton of insects and other arthropods is a remarkably versatile material with a complex multilayer structure. We made use of the ability to isolate cuticle synthesizing cells in relatively pure form by dissecting pupal wings and we used RNAseq to identify genes expressed during the formation of the adult wing cuticle. We observed dramatic changes in gene expression during cuticle deposition, and combined with transmission electron microscopy, we were able to identify candidate genes for the deposition of the different cuticular layers. Among genes of interest that dramatically change their expression during the cuticle deposition program are ones that encode cuticle proteins, ZP domain proteins, cuticle modifying proteins and transcription factors, as well as genes of unknown function. A striking finding is that mutations in a number of genes that are expressed almost exclusively during the deposition of the envelope (the thin outermost layer that is deposited first) result in gross defects in the procuticle (the thick chitinous layer that is deposited last). An attractive hypothesis to explain this is that the deposition of the different cuticle layers is not independent with the envelope instructing the formation of later layers. Alternatively, some of the genes expressed during the deposition of the envelope could form a platform that is essential for the deposition of all cuticle layers.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.