ArticlePeerJ2016
Development of an angiogenesis-promoting microvesicle-alginate-polycaprolactone composite graft for bone tissue engineering applications.
Article in PeerJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- Review
- Anatomical implant region - a critical determinant for the osteogenic potency of small extracellular vesicles and rhBMP-2.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2025Article
- Role of Mesenchymal Stem Cell Exosomes and Injectable Platelet Rich Fibrin on Structure and Function of Submandibular Salivary Gland of Aged Albino Rats.The Saudi dental journal · 2025Article
- Extracellular vesicles: essential agents in critical bone defect repair and therapeutic enhancement.Molecular biology reports · 2025Review
- Extracellular vesicle-functionalized bioactive scaffolds for bone regeneration.Asian journal of pharmaceutical sciences · 2024Review
- Histological and Immunohistochemical Evaluation of Rh-BMP2: Effect on Gingival Healing Acceleration and Proliferation of Human Epithelial Cells.Life (Basel, Switzerland) · 2024Article
- Effect of rh-BMP-2 in the Initiation of Neovascularization in Human Gingival Tissue: A Split-Mouth Clinical Study.Life (Basel, Switzerland) · 2023Article
- Review
- Development of Surgically Transplantable Parathyroid Hormone-Releasing Microbeads.Biomedicines · 2022Article
- Delayed Supplementation Strategy of Extracellular Vesicles from Adipose-Derived Mesenchymal Stromal Cells with Improved Proregenerative Efficiency in a Fat Transplantation Model.Stem cells international · 2022Article
- Mesenchymal Stem Cell Senescence and Osteogenesis.Medicina (Kaunas, Lithuania) · 2021Review
- Bioactive Scaffolds Integrated with Liposomal or Extracellular Vesicles for Bone Regeneration.Bioengineering (Basel, Switzerland) · 2021Review
- The Application of Polycaprolactone in Three-Dimensional Printing Scaffolds for Bone Tissue Engineering.Polymers · 2021Review
- Review
- Innovative Molecular and Cellular Therapeutics in Cleft Palate Tissue Engineering.Tissue engineering. Part B, Reviews · 2021Review
- Mesenchymal stromal cell-derived extracellular vesicles for bone regeneration therapy.Bone reports · 2021Review
- 3D-microtissue derived secretome as a cell-free approach for enhanced mineralization of scaffolds in the chorioallantoic membrane model.Scientific reports · 2021Article
- Senescent Mesenchymal Stem Cells: Disease Mechanism and Treatment Strategy.Current molecular biology reports · 2020Article
- Functional Validation of a New Alginate-based Hydrogel Scaffold Combined with Mesenchymal Stem Cells in a Rat Hard Palate Cleft Model.Plastic and reconstructive surgery. Global open · 2020Article
- An Alginate-Based Hydrogel with a High Angiogenic Capacity and a High Osteogenic Potential.BioResearch open access · 2020Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the major challenges of bone tissue engineering applications is to construct a fully vascularized implant that can adapt to hypoxic environments in vivo. The incorporation of proangiogenic factors into scaffolds is a widely accepted method of achieving this goal. Recently, the proangiogenic potential of mesenchymal stem cell-derived microvesicles (MSC-MVs) has been confirmed in several studies. In the present study, we incorporated MSC-MVs into alginate-polycaprolactone (PCL) constructs that had previously been developed for bone tissue engineering applications, with the aim of promoting angiogenesis and bone regeneration. MSC-MVs were first isolated from the supernatant of rat bone marrow-derived MSCs and characterized by scanning electron microscopic, confocal microscopic, and flow cytometric analyses. The proangiogenic potential of MSC-MVs was demonstrated by the stimulation of tube formation of human umbilical vein endothelial cells in vitro. MSC-MVs and osteodifferentiated MSCs were then encapsulated with alginate and seeded onto porous three-dimensional printed PCL scaffolds. When combined with osteodifferentiated MSCs, the MV-alginate-PCL constructs enhanced vessel formation and tissue-engineered bone regeneration in a nude mouse subcutaneous bone formation model, as demonstrated by micro-computed tomographic, histological, and immunohistochemical analyses. This MV-alginate-PCL construct may offer a novel, proangiogenic, and cost-effective option for bone tissue engineering.
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