ArticlePeerJ2016
Optimization of viral resuspension methods for carbon-rich soils along a permafrost thaw gradient.
Article in PeerJ, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Virome DNA stable isotope probing reveals diverse active soil virus communities across ecosystem contexts.mSystems · 2026Article
- Disentangling production and persistence of extracellular virions in grassland soils with SIP-viromics.mSystems · 2026Article
- DNA Viral Size Fraction Metagenomics for Human Stool Samples.Journal of visualized experiments : JoVE · 2026Article
- Viromics approaches for the study of viral diversity and ecology in microbiomes.Nature reviews. Genetics · 2026Review
- Population ecology and biogeochemical implications of ssDNA and dsDNA viruses along a permafrost thaw gradient.Nature communications · 2025Article
- Soil viruses drive carbon turnover during subtropical secondary forest succession.Frontiers in microbiology · 2025Article
- Benchmarking bioinformatic virus identification tools using real-world metagenomic data across biomes.Genome biology · 2024Article
- Viral potential to modulate microbial methane metabolism varies by habitat.Nature communications · 2024Article
- Activity of novel virus families infecting soil nitrifiers is concomitant with host niche differentiation.The ISME journal · 2024Article
- Breaking the Ice: A Review of Phages in Polar Ecosystems.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Zea mays genotype influences microbial and viral rhizobiome community structure.ISME communications · 2023Article
- A subset of viruses thrives following microbial resuscitation during rewetting of a seasonally dry California grassland soil.Nature communications · 2023Article
- Meeting report: The first soil viral workshop 2022.Virus research · 2023Article
- Ecogenomics reveals viral communities across the Challenger Deep oceanic trench.Communications biology · 2022Article
- An Appraisal of Bacteriophage Isolation Techniques from Environment.Microbial ecology · 2022Review
- RNA-viromics reveals diverse communities of soil RNA viruses with the potential to affect grassland ecosystems across multiple trophic levels.ISME communications · 2022Article
- Distribution Characteristics of Soil Viruses Under Different Precipitation Gradients on the Qinghai-Tibet Plateau.Frontiers in microbiology · 2022Article
- Article
- Minnesota peat viromes reveal terrestrial and aquatic niche partitioning for local and global viral populations.Microbiome · 2021Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Permafrost stores approximately 50% of global soil carbon (C) in a frozen form; it is thawing rapidly under climate change, and little is known about viral communities in these soils or their roles in C cycling. In permafrost soils, microorganisms contribute significantly to C cycling, and characterizing them has recently been shown to improve prediction of ecosystem function. In other ecosystems, viruses have broad ecosystem and community impacts ranging from host cell mortality and organic matter cycling to horizontal gene transfer and reprogramming of core microbial metabolisms. Here we developed an optimized protocol to extract viruses from three types of high organic-matter peatland soils across a permafrost thaw gradient (palsa, moss-dominated bog, and sedge-dominated fen). Three separate experiments were used to evaluate the impact of chemical buffers, physical dispersion, storage conditions, and concentration and purification methods on viral yields. The most successful protocol, amended potassium citrate buffer with bead-beating or vortexing and BSA, yielded on average as much as 2-fold more virus-like particles (VLPs) g(-1) of soil than other methods tested. All method combinations yielded VLPs g(-1) of soil on the 10(8) order of magnitude across all three soil types. The different storage and concentration methods did not yield significantly more VLPs g(-1) of soil among the soil types. This research provides much-needed guidelines for resuspending viruses from soils, specifically carbon-rich soils, paving the way for incorporating viruses into soil ecology studies.
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