Evidence map›Paper›PMID 27231629›Full record

ArticleBiomedical optics express2016

Third harmonic generation imaging for fast, label-free pathology of human brain tumors.

N V Kuzmin, P Wesseling, P C de Witt Hamer, D P Noske, G D Galgano, H D Mansvelder, J C Baayen, M L Groot

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.

0numbers the graph read from it
0cells of the map it votes in
43citing papers in PubMed
13.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

43 citing papers in PubMed, 85 citations in OpenAlex.

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  12. Current Landscape of Advanced Imaging Tools for Pathology Diagnostics.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2024
    Review
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  16. Review
  17. Roadmap on Label-Free Super-Resolution Imaging.Laser & photonics reviews · 2023
    Article
  18. Review
  19. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

N V KuzminLaserLab Amsterdam, VU University, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands; Neuroscience Campus Amsterdam, VU University, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
P WesselingDept. of Pathology, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands; Dept. of Pathology, Radboud University Medical Center, Geert Grooteplein Zuid, 6525 GA Nijmegen, The Netherlands; Amsterdam Brain Tumor Center, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands.
P C de Witt HamerDept. of Neurosurgery, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands; Amsterdam Brain Tumor Center, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands.
D P NoskeDept. of Neurosurgery, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands; Amsterdam Brain Tumor Center, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands.
G D GalganoLaserLab Amsterdam, VU University, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands.
H D MansvelderNeuroscience Campus Amsterdam, VU University, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
J C BaayenDept. of Neurosurgery, VU University Medical Center, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands.
M L GrootLaserLab Amsterdam, VU University, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands; Neuroscience Campus Amsterdam, VU University, De Boelelaan 1085, 1081 HV Amsterdam, The Netherlands.
Amsterdam UMC Location VUmc · NLAmsterdam Neuroscience · NLVrije Universiteit Amsterdam · NL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In brain tumor surgery, recognition of tumor boundaries is key. However, intraoperative assessment of tumor boundaries by the neurosurgeon is difficult. Therefore, there is an urgent need for tools that provide the neurosurgeon with pathological information during the operation. We show that third harmonic generation (THG) microscopy provides label-free, real-time images of histopathological quality; increased cellularity, nuclear pleomorphism, and rarefaction of neuropil in fresh, unstained human brain tissue could be clearly recognized. We further demonstrate THG images taken with a GRIN objective, as a step toward in situ THG microendoscopy of tumor boundaries. THG imaging is thus a promising tool for optical biopsies.

Indexed as

(170.1530) Cell analysis(170.2150) Endoscopic imaging(170.4730) Optical pathology(170.6935) Tissue characterization(180.4315) Nonlinear microscopy(190.4160) Multiharmonic generation

Identifiers

PMID27231629
PMCPMC4871089
OpenAlexW2336635241

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.