ArticleBiomedical optics express2016
Using optical trap to measure the refractive index of a single animal virus in culture fluid with high precision.
Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 61 citations in OpenAlex.
- Dynamics of viral self-assembly, viral genome packaging, and virus-cell interactions studied by optical tweezers.European biophysics journal : EBJ · 2026Review
- A Refractive Index-Based Dual-Band Metamaterial Sensor Design and Analysis for Biomedical Sensing Applications.Sensors (Basel, Switzerland) · 2025Article
- Silicon Microring Resonator Biosensor for Detection of Nucleocapsid Protein of SARS-CoV-2.Sensors (Basel, Switzerland) · 2024Article
- Deep learning-based size prediction for optical trapped nanoparticles and extracellular vesicles from limited bandwidth camera detection.Biomedical optics express · 2024Article
- Optical Tweezers to Study Viruses.Sub-cellular biochemistry · 2024Review
- Mirror-Enhanced Plasmonic Nanoaperture for Ultrahigh Optical Force Generation with Minimal Heat Generation.Nano letters · 2023Article
- Highly sensitive label-free biosensor: graphene/CaFScientific reports · 2023Article
- Article
- Optical Biosensors for the Diagnosis of COVID-19 and Other Viruses-A Review.Diagnostics (Basel, Switzerland) · 2023Review
- Comparison of interferometric light microscopy with nanoparticle tracking analysis for the study of extracellular vesicles and bacteriophages.Journal of extracellular biology · 2023Article
- A feasibility study of different commercially available serum-free mediums to enhance lentivirus and adeno-associated virus production in HEK 293 suspension cells.Cytotechnology · 2022Article
- Exploring Masses and Internal Mass Distributions of Single Carboxysomes in Free Solution Using Fluorescence and Interferometric Scattering in an Anti-Brownian Trap.The journal of physical chemistry. B · 2022Article
- 100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales.Nature communications · 2022Article
- On-Chip Optical Detection of Viruses: A Review.Advanced photonics research · 2021Article
- Nanostructured ZnO and ZnO: Pd with MXene overlayer SPR biosensors.Optical and quantum electronics · 2021Article
- Demonstration of a Label-Free and Low-Cost Optical Cavity-Based Biosensor Using Streptavidin and C-Reactive Protein.Biosensors · 2020Article
- Determination of refractive index, size, and solid content of monodisperse polystyrene microsphere suspensions for the characterization of optical phantoms.Biomedical optics express · 2020Article
- A Novel Semiconductor-Based Flow Cytometer with Enhanced Light-Scatter Sensitivity for the Analysis of Biological Nanoparticles.Scientific reports · 2019Article
- Back-focal-plane interferometric detection of nanoparticles in spatially confined microfluidic channels.The Review of scientific instruments · 2019Article
- Selective Isolation of Retroviruses from Extracellular Vesicles by Intact Virion Immunoprecipitation.Bio-protocol · 2018Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The refractive index (RI) is a fundamental parameter of materials that can be used to distinguish and sort materials of different nature. Although the RI of a virus is required for many optics-based biosensing applications, RIs of animal viruses have never been measured. Here we have developed a technique that can measure the RI of individual viruses in aqueous media with high precision. This technique is based on optical trapping of single virions and works by relating the size and RI of a single virus to the stiffness of an optical trap. We have derived an analytic expression to quantitatively describe the optical trapping of these particles. We have validated this equation using nanoparticles of known RI, and measured the RI of individual human immunodeficiency viruses type-1, which yielded a value of 1.42 at 830 nm with less than 2% coefficient of variation. This value is much lower than the RI typically assumed for viruses, but very close to that of 2.0 M sucrose solution in water. To the best of our knowledge, this is the first report on the experimental measurement of the RI for a single animal virus in aqueous media. This technique does not require prior knowledge on the diameter of the nanoparticles, and can be applied to other viruses or nanoparticles for accurate measurement of RI that is critical for the label-free detection of these particles in various settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.