Evidence map›Paper›PMID 27231594›Full record

ArticleBiomedical optics express2016

Multifocus microscopy with precise color multi-phase diffractive optics applied in functional neuronal imaging.

Sara Abrahamsson, Rob Ilic, Jan Wisniewski, Brian Mehl, Liya Yu, Lei Chen, Marcelo Davanco, Laura Oudjedi, Jean-Bernard Fiche, Bassam Hajj and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed.

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  18. Computational multifocal microscopy.Biomedical optics express · 2018
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Sara AbrahamssonHHMI and Lulu and Anthony Wang Laboratory of Neural Circuits and Behavior, The Rockefeller University, New York, NY 10065, USA; Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Rob IlicCenter for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Jan WisniewskiTranscription Imaging Consortium, HHMI Janelia Research Campus, Ashburn, VA 20147, USA.
Brian MehlTranscription Imaging Consortium, HHMI Janelia Research Campus, Ashburn, VA 20147, USA.
Liya YuCenter for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Lei ChenCenter for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Marcelo DavancoCenter for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Laura OudjediCentre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
Jean-Bernard FicheCentre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
Bassam HajjTranscription Imaging Consortium, HHMI Janelia Research Campus, Ashburn, VA 20147, USA; Laboratoire Physico Chimie, Institut Curie, CNRS UMR 168, Université Pierre et Marie Curie-Paris 6, 11 rue Pierre et Marie Curie, 75005 Paris France.
Xin JinHHMI and Lulu and Anthony Wang Laboratory of Neural Circuits and Behavior, The Rockefeller University, New York, NY 10065, USA.
Joan PulupaLaboratory of Cellular Biophysics, The Rockefeller University, New York, NY 10065, USA.
Christine ChoHHMI and Lulu and Anthony Wang Laboratory of Neural Circuits and Behavior, The Rockefeller University, New York, NY 10065, USA.
Mustafa MirTranscription Imaging Consortium, HHMI Janelia Research Campus, Ashburn, VA 20147, USA; University of California, Berkeley, CA 94720, USA.
Mohamed El BeheiryTranscription Imaging Consortium, HHMI Janelia Research Campus, Ashburn, VA 20147, USA; Laboratoire Physico Chimie, Institut Curie, CNRS UMR 168, Université Pierre et Marie Curie-Paris 6, 11 rue Pierre et Marie Curie, 75005 Paris France.
Xavier DarzacqTranscription Imaging Consortium, HHMI Janelia Research Campus, Ashburn, VA 20147, USA; University of California, Berkeley, CA 94720, USA.
Marcelo NollmannCentre de Biochimie Structurale, CNRS UMR5048, INSERM U1054, Université de Montpellier, 29 rue de Navacelles, 34090 Montpellier, France.
Maxime DahanTranscription Imaging Consortium, HHMI Janelia Research Campus, Ashburn, VA 20147, USA; Laboratoire Physico Chimie, Institut Curie, CNRS UMR 168, Université Pierre et Marie Curie-Paris 6, 11 rue Pierre et Marie Curie, 75005 Paris France.
Carl WuTranscription Imaging Consortium, HHMI Janelia Research Campus, Ashburn, VA 20147, USA.
Timothée LionnetTranscription Imaging Consortium, HHMI Janelia Research Campus, Ashburn, VA 20147, USA.
J Alexander LiddleCenter for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Cornelia I BargmannHHMI and Lulu and Anthony Wang Laboratory of Neural Circuits and Behavior, The Rockefeller University, New York, NY 10065, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Multifocus microscopy (MFM) allows high-resolution instantaneous three-dimensional (3D) imaging and has been applied to study biological specimens ranging from single molecules inside cells nuclei to entire embryos. We here describe pattern designs and nanofabrication methods for diffractive optics that optimize the light-efficiency of the central optical component of MFM: the diffractive multifocus grating (MFG). We also implement a "precise color" MFM layout with MFGs tailored to individual fluorophores in separate optical arms. The reported advancements enable faster and brighter volumetric time-lapse imaging of biological samples. In live microscopy applications, photon budget is a critical parameter and light-efficiency must be optimized to obtain the fastest possible frame rate while minimizing photodamage. We provide comprehensive descriptions and code for designing diffractive optical devices, and a detailed methods description for nanofabrication of devices. Theoretical efficiencies of reported designs is ≈90% and we have obtained efficiencies of > 80% in MFGs of our own manufacture. We demonstrate the performance of a multi-phase MFG in 3D functional neuronal imaging in living C. elegans.

Indexed as

(050.1970) Diffractive optics(110.4190) Multiple imaging(180.2520) Fluorescence microscopy(180.6900) Three-dimensional microscopy(260.5430) Polarization

Identifiers

PMID27231594
PMCPMC4866461

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.