ArticleThe Journal of biological chemistry2016
A Cyclic di-GMP-binding Adaptor Protein Interacts with Histidine Kinase to Regulate Two-component Signaling.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- A cyclic di-GMP-binding adaptor protein interacts with a N5-glutamine methyltransferase to regulate the pathogenesis in Xanthomonas citri subsp. citri.Molecular plant pathology · 2024Article
- PQS and pyochelin inMicrobiology spectrum · 2024Article
- Functional diversity of c-di-GMP receptors in prokaryotic and eukaryotic systems.Cell communication and signaling : CCS · 2023Review
- Blue light promotes zero-valent sulfur production in a deep-sea bacterium.The EMBO journal · 2023Article
- Evolution of cyclic di-GMP signalling on a short and long term time scale.Microbiology (Reading, England) · 2023Article
- Pseudomonas aeruginosa biofilm dispersion by the mouse antimicrobial peptide CRAMP.Veterinary research · 2022Article
- Structural Conservation and Diversity of PilZ-Related Domains.Journal of bacteriology · 2020Article
- Regulation of Exopolysaccharide Production by ProE, a Cyclic-Di-GMP Phosphodiesterase inFrontiers in microbiology · 2020Article
- AmrZ Regulates Swarming Motility Through Cyclic di-GMP-Dependent Motility Inhibition and Controlling Pel Polysaccharide Production inFrontiers in microbiology · 2019Article
- Identification of Cbp1, a c-di-GMP Binding Chemoreceptor inFrontiers in microbiology · 2019Article
- Structural analyses unravel the molecular mechanism of cyclic di-GMP regulation of bacterial chemotaxis via a PilZ adaptor protein.The Journal of biological chemistry · 2018Article
- Identification and Characterization of c-di-GMP Metabolic Enzymes ofFrontiers in microbiology · 2018Article
- Cyclic-di-GMP regulation of virulence in bacterial pathogens.Wiley interdisciplinary reviews. RNA · 2018Review
- Two-component systems required for virulence in Pseudomonas aeruginosa.FEMS microbiology letters · 2017Review
- Discovery of a Novel Nitric Oxide Binding Protein and Nitric-Oxide-Responsive Signaling Pathway in Pseudomonas aeruginosa.ACS infectious diseases · 2017Article
- Divide and conquer: the Pseudomonas aeruginosa two-component hybrid SagS enables biofilm formation and recalcitrance of biofilm cells to antimicrobial agents via distinct regulatory circuits.Environmental microbiology · 2017Article
- BrothFrontiers in microbiology · 2016Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
The bacterial messenger cyclic di-GMP (c-di-GMP) binds to a diverse range of effectors to exert its biological effect. Despite the fact that free-standing PilZ proteins are by far the most prevalent c-di-GMP effectors known to date, their physiological function and mechanism of action remain largely unknown. Here we report that the free-standing PilZ protein PA2799 from the opportunistic pathogen Pseudomonas aeruginosa interacts directly with the hybrid histidine kinase SagS. We show that PA2799 (named as HapZ: histidine kinase associated PilZ) binds directly to the phosphoreceiver (REC) domain of SagS, and that the SagS-HapZ interaction is further enhanced at elevated c-di-GMP concentration. We demonstrate that binding of HapZ to SagS inhibits the phosphotransfer between SagS and the downstream protein HptB in a c-di-GMP-dependent manner. In accordance with the role of SagS as a motile-sessile switch and biofilm growth factor, we show that HapZ impacts surface attachment and biofilm formation most likely by regulating the expression of a large number of genes. The observations suggest a previously unknown mechanism whereby c-di-GMP mediates two-component signaling through a PilZ adaptor protein.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.