ArticleThe Journal of biological chemistry2016
Discovery of a Novel Alginate Lyase from Nitratiruptor sp. SB155-2 Thriving at Deep-sea Hydrothermal Vents and Identification of the Residues Responsible for Its Heat Stability.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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45 citing papers in PubMed.
- Alginate-Degrading Modes, Oligosaccharide-Yielding Properties, and Potential Applications of a Novel Bacterial Multifunctional Enzyme, Aly16-1.Microorganisms · 2024Article
- Genome Analysis of a Potential NovelMarine drugs · 2024Article
- A Novel Cold-Adapted and High-Alkaline Alginate Lyase with Potential for Alginate Oligosaccharides Preparation.Molecules (Basel, Switzerland) · 2023Article
- Article
- Biotransformation of gallate esters by a pH-stable tannase of mangrove-derived yeastFrontiers in molecular biosciences · 2023Article
- Cloning and Characterization of a Novel Endo-Type Metal-Independent Alginate Lyase from the Marine BacteriaMarine drugs · 2022Article
- Alginate Lyases from Marine Bacteria: An Enzyme Ocean for Sustainable Future.Molecules (Basel, Switzerland) · 2022Review
- Marine microbial enzymes for the production of algal oligosaccharides and its bioactive potential for application as nutritional supplements.Folia microbiologica · 2022Review
- Biochemical Characterization and Cold-Adaption Mechanism of a PL-17 Family Alginate Lyase Aly23 from Marine BacteriumMarine drugs · 2022Article
- Evolving strategies for marine enzyme engineering: recent advances on the molecular modification of alginate lyase.Marine life science & technology · 2022Review
- Improving the thermostability of alginate lyase FlAlyA with high expression by computer-aided rational design for industrial preparation of alginate oligosaccharides.Frontiers in bioengineering and biotechnology · 2022Article
- Enzymatic depolymerization of alginate by two novel thermostable alginate lyases fromFrontiers in plant science · 2022Article
- Structure Characteristics, Biochemical Properties, and Pharmaceutical Applications of Alginate Lyases.Marine drugs · 2021Review
- Oxidative Stress Response inPathogens (Basel, Switzerland) · 2021Review
- Bacterial alginate metabolism: an important pathway for bioconversion of brown algae.Biotechnology for biofuels · 2021Review
- Characterization of a New Biofunctional, Exolytic Alginate Lyase fromMarine drugs · 2021Article
- Expression and Characterization of a Cold-Adapted Alginate Lyase with Exo/Endo-Type Activity from a Novel Marine BacteriumMarine drugs · 2021Article
- Specificities and Synergistic Actions of Novel PL8 and PL7 Alginate Lyases from the Marine FungusJournal of fungi (Basel, Switzerland) · 2021Article
- Secretory Expression of an Alkaline Alginate Lyase With Heat Recovery Property inFrontiers in microbiology · 2021Article
- Recent progress on engineering microbial alginate lyases towards their versatile role in biotechnological applications.Folia microbiologica · 2020Review
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4 authors.
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Abstract
Extremophiles are expected to represent a source of enzymes having unique functional properties. The hypothetical protein NIS_0185, termed NitAly in this study, was identified as an alginate lyase-homolog protein in the genomic database of ϵ-Proteobacteria Nitratiruptor sp. SB155-2, which was isolated from deep-sea hydrothermal vents at a water depth of 1,000 m. Among the characterized alginate lyases in the polysaccharide lyase family 7 (PL-7), the amino acid sequence of NitAly showed the highest identity (39%) with that of red alga Pyropia yezoensis alginate lyase PyAly. Recombinant NitAly (rNitAly) was successfully expressed in Escherichia coli Purified rNitAly degraded alginate in an endolytic manner. Among alginate block types, polyM was preferable to polyG and polyMG as a substrate, and its end degradation products were mainly tri-, tetra-, and penta-saccharides. The optimum temperature and pH values were 70 °C and around 6, respectively. A high concentration of NaCl (0.8-1.4 m) was required for maximum activity. In addition, a 50% loss of activity was observed after incubation at 67 °C for 30 min. Heat stability was decreased in the presence of 5 mm DTT, and Cys-80 and Cys-232 were identified as the residues responsible for heat stability but not lyase activity. Introducing two cysteines into PyAly based on homology modeling using Pseudomonas aeruginosa alginate lyase PA1167 as the template enhanced its heat stability. Thus, NitAly is a functional alginate lyase, with its unique optimum conditions adapted to its environment. These insights into the heat stability of NitAly could be applied to improve that of other PL-7 alginate lyases.
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