ArticleBMC nephrology2016
Gas6 protein: its role in cardiovascular calcification.
Article in BMC nephrology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Vitamin K Supplementation to Improve Vascular Stiffness in CKD: The K4Kidneys Randomized Controlled Trial.Journal of the American Society of Nephrology : JASN · 2020Trial
- Targeting calciphylaxis: the emergence of vitamin K as a potential treatment.Clinical kidney journal · 2026Review
- Longevity mechanisms in cardiac aging: exploring calcium dysregulation and senescence.Biogerontology · 2025Review
- The biological responses of vitamin K2: A comprehensive review.Food science & nutrition · 2023Review
- Vascular Calcification: In Vitro Models under the Magnifying Glass.Biomedicines · 2022Review
- Vitamin K - sources, physiological role, kinetics, deficiency, detection, therapeutic use, and toxicity.Nutrition reviews · 2022Review
- The two facets of receptor tyrosine kinase in cardiovascular calcification-can tyrosine kinase inhibitors benefit cardiovascular system?Frontiers in cardiovascular medicine · 2022Review
- Ginsenoside Rb1 attenuates age-associated vascular impairment by modulating the Gas6 pathway.Pharmaceutical biology · 2021Article
- Molecular Mechanism of Vitamin K2 Protection against Amyloid-β-Induced Cytotoxicity.Biomolecules · 2021Article
- Sirtuin-1 and Its Relevance in Vascular Calcification.International journal of molecular sciences · 2020Review
- Vitamin K Dependent Proteins in Kidney Disease.International journal of molecular sciences · 2019Review
- The Effects of Parenteral K1 Administration in Pseudoxanthoma Elasticum Patients Versus Controls. A Pilot Study.Frontiers in medicine · 2018Article
- Vitamin K2 inhibits rat vascular smooth muscle cell calcification by restoring the Gas6/Axl/Akt anti-apoptotic pathway.Molecular and cellular biochemistry · 2017Article
- Arterial Calcification in Diabetes Mellitus: Preclinical Models and Translational Implications.Arteriosclerosis, thrombosis, and vascular biology · 2017Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCardiovascular calcifications can be prevented by vitamin K and are accelerated by vitamin K antagonists. These effects are believed to be mainly mediated by the vitamin K-dependent matrix Gla protein. Another vitamin K-dependent protein, Gas6, is also expressed in vascular smooth muscle cells (VSMC). In vitro Gas6 expression was shown to be regulated in VSMC calcification and apoptotic processes.
methodsWe investigated the role of Gas6 in vitro using VSMC cultures and in vivo in young and old Gas6-deficient (Gas6(-/-)) and wildtype (WT) mice. In addition, Gas6(-/-) and WT mice were challenged by (a) warfarin administration, (b) uninephrectomy (UniNX) plus high phosphate diet, or (c) UniNX plus high phosphate plus electrocautery of the residual kidney.
resultsIn vitro VSMC from WT and Gas6(-/-) mice exposed to warfarin showed increased apoptosis and calcified similarly. In vivo, aortic, cardiac and renal calcium content in all groups was similar, except for a lower cardiac calcium content in Gas6(-/-) mice (group a). Von Kossa staining revealed small vascular calcifications in both WT and Gas6(-/-) mice (groups a-c). In aging, non-manipulated mice, no significant differences in vascular calcification were identified between Gas6(-/-) and WT mice. Gas6(-/-) mice exhibited no upregulation of matrix Gla protein in any group. Cardiac output was similar in all treatment groups.
conclusionsTaken together, in our study Gas6 fails to aggravate calcification against the previous assumption.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.