ArticleScientific reports2016
Pathway Compartmentalization in Peroxisome of Saccharomyces cerevisiae to Produce Versatile Medium Chain Fatty Alcohols.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 89 citations in OpenAlex.
- ML-enhanced peroxisome capacity enables compartmentalization of multienzyme pathway.Nature chemical biology · 2025Article
- Harnessing organelle engineering to facilitate biofuels and biochemicals production in yeast.Journal of microbiology (Seoul, Korea) · 2025Review
- A Rewired NADPH-Dependent Redox Shuttle for Testing Peroxisomal Compartmentalization of Synthetic Metabolic Pathways inMicroorganisms · 2024Article
- Advanced metabolic Engineering strategies for the sustainable production of free fatty acids and their derivatives using yeast.Journal of biological engineering · 2024Review
- Cell factories for biosynthesis of D-glucaric acid: a fusion of static and dynamic strategies.World journal of microbiology & biotechnology · 2024Review
- Overcoming barriers to medium-chain fatty alcohol production.Current opinion in biotechnology · 2024Review
- Towards using bacterial microcompartments as a platform for spatial metabolic engineering in the industrially important and metabolically versatileFrontiers in bioengineering and biotechnology · 2024Article
- Peroxisomal metabolic coupling improves fatty alcohol production from sole methanol in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Key enzymes involved in the utilization of fatty acids byFrontiers in microbiology · 2023Review
- Spatial-temporal regulation of fatty alcohol biosynthesis in yeast.Biotechnology for biofuels and bioproducts · 2022Article
- Functional diversity and metabolic engineering of plant-specialized metabolites.Life metabolism · 2022Review
- Engineering for life in toxicity: Key to industrializing microbial synthesis of high energy density fuels.Engineering microbiology · 2022Review
- Microbial synthesis of long-chain α-alkenes from methanol by engineering Pichia pastoris.Bioresources and bioprocessing · 2022Article
- Microbial engineering to produce fatty alcohols and alkanes.Journal of industrial microbiology & biotechnology · 2021Review
- Peroxisomes: A New Hub for Metabolic Engineering in Yeast.Frontiers in bioengineering and biotechnology · 2021Article
- Transforming yeast peroxisomes into microfactories for the efficient production of high-value isoprenoids.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Harnessing sub-organelle metabolism for biosynthesis of isoprenoids in yeast.Synthetic and systems biotechnology · 2020Review
- Biosynthesis of Fatty Alcohols in Engineered Microbial Cell Factories: Advances and Limitations.Frontiers in bioengineering and biotechnology · 2020Review
- An engineered fatty acid synthase combined with a carboxylic acid reductase enables de novo production of 1-octanol inBiotechnology for biofuels · 2018Article
- Microbial synthesis of medium-chain chemicals from renewables.Nature biotechnology · 2017Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fatty alcohols are value-added chemicals and important components of a variety of industries, which have a >3 billion-dollar global market annually. Long chain fatty alcohols (>C12) are mainly used in surfactants, lubricants, detergents, pharmaceuticals and cosmetics while medium chain fatty alcohols (C6-C12) could be used as diesel-like biofuels. Microbial production of fatty alcohols from renewable feedstock stands as a promising strategy to enable sustainable supply of fatty alcohols. In this study, we report, for the first time, that medium chain fatty alcohols could be produced in yeast via targeted expression of a fatty acyl-CoA reductase (TaFAR) in the peroxisome of Saccharomyces cerevisiae. By tagging TaFAR enzyme with peroxisomal targeting signal peptides, the TaFAR could be compartmentalized into the matrix of the peroxisome to hijack the medium chain fatty acyl-CoA generated from the beta-oxidation pathway and convert them to versatile medium chain fatty alcohols (C10 &C12). The overexpression of genes encoding PEX7 and acetyl-CoA carboxylase further improved fatty alcohol production by 1.4-fold. After medium optimization in fed-batch fermentation using glucose as the sole carbon source, fatty alcohols were produced at 1.3 g/L, including 6.9% 1-decanol, 27.5% 1-dodecanol, 2.9% 1-tetradecanol and 62.7% 1-hexadecanol. This work revealed that peroxisome could be engineered as a compartmentalized organelle for producing fatty acid-derived chemicals in S. cerevisiae.
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