ArticleNature communications2016
A dual molecular analogue tuner for dissecting protein function in mammalian cells.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- Mapping early PRC2 nucleation sites upon Suz12 reintroduction reveals features ofbioRxiv : the preprint server for biology · 2025Article
- Manipulation of targeted protein degradation in plant biology.Biochemical Society transactions · 2025Review
- Rapid depletion of target proteins in plants by an inducible protein degradation system.The Plant cell · 2024Article
- Methodology to Create Auxin-Inducible Degron Tagging System to Control Expression of a Target Protein in Mammalian Cell Lines.Bio-protocol · 2024Article
- Evidence that tissue recoil in the earlyMolecular biology of the cell · 2023Article
- A robust dual gene ON-OFF toggle directed by two independent promoter-degron pairs.Journal of cell science · 2023Article
- Strong and tunable anti-CRISPR/Cas activities in plants.Plant biotechnology journal · 2022Article
- Controlling CRISPR with small molecule regulation for somatic cell genome editing.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Review
- A One-step strategy to target essential factors with auxin-inducible degron system in mouse embryonic stem cells.Frontiers in cell and developmental biology · 2022Article
- One-step multiplex toolkit for efficient generation of conditional gene silencing human cell lines.Molecular biology of the cell · 2021Article
- Article
- Evaluation of the OsTIR1 and AtAFB2 AID Systems for Genome Architectural Protein Degradation in Mammalian Cells.Frontiers in molecular biosciences · 2021Article
- Adaptation of the AID system for stem cell and transgenic mouse research.Stem cell research · 2020Article
- ARF-AID: A Rapidly Inducible Protein Degradation System That Preserves Basal Endogenous Protein Levels.Current protocols in molecular biology · 2020Article
- Isolation of gene-edited cells via knock-in of short glycophosphatidylinositol-anchored epitope tags.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Loss-of-function studies are fundamental for dissecting gene function. Yet, methods to rapidly and effectively perturb genes in mammalian cells, and particularly in stem cells, are scarce. Here we present a system for simultaneous conditional regulation of two different proteins in the same mammalian cell. This system harnesses the plant auxin and jasmonate hormone-induced degradation pathways, and is deliverable with only two lentiviral vectors. It combines RNAi-mediated silencing of two endogenous proteins with the expression of two exogenous proteins whose degradation is induced by external ligands in a rapid, reversible, titratable and independent manner. By engineering molecular tuners for NANOG, CHK1, p53 and NOTCH1 in mammalian stem cells, we have validated the applicability of the system and demonstrated its potential to unravel complex biological processes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.