ArticleScientific reports2016
High Production of 3-Hydroxypropionic Acid in Klebsiella pneumoniae by Systematic Optimization of Glycerol Metabolism.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 95 citations in OpenAlex.
- Redox-based dual-substrate fermentation enhances sustainable production of 3-hydroxypropionic acid by recombinant E. coli.Applied microbiology and biotechnology · 2026Article
- Advances in metabolic engineering and fermentation for 3-hydroxypropionic acid biosynthesis: a comprehensive review.World journal of microbiology & biotechnology · 2025Review
- Metabolic and fermentation engineering of Klebsiella Pneumoniae for high-level production of 3-hydroxypropionic acid.Archives of microbiology · 2025Article
- Insufficient Acetyl-CoA Pool Restricts the Phototrophic Production of Organic Acids in Model Cyanobacteria.International journal of molecular sciences · 2024Review
- Construction of cascade circuits for dynamic temporal regulation and its application to PHB production.Biotechnology for biofuels and bioproducts · 2023Article
- Engineering 3-Hydroxypropionic Acid Production from Glucose inJournal of fungi (Basel, Switzerland) · 2023Article
- Metabolic engineering to improve production of 3-hydroxypropionic acid from corn-stover hydrolysate in Aspergillus species.Biotechnology for biofuels and bioproducts · 2023Article
- Repurposing of waste PET by microbial biotransformation to functionalized materials for additive manufacturing.Journal of industrial microbiology & biotechnology · 2023Article
- Production of 3-Hydroxypropionic Acid from Renewable Substrates by Metabolically Engineered Microorganisms: A Review.Molecules (Basel, Switzerland) · 2023Review
- EngineeringFrontiers in bioengineering and biotechnology · 2023Article
- A Gene Circuit Combining the Endogenous I-E Type CRISPR-Cas System and a Light Sensor to Produce Poly-β-Hydroxybutyric Acid Efficiently.Biosensors · 2022Article
- Discovering interrelated natural mutations of efflux pump KmrA from Klebsiella pneumoniae that confer increased multidrug resistance.Protein science : a publication of the Protein Society · 2022Article
- A curated collection ofGenome research · 2022Article
- Engineering Glucose-to-Glycerol Pathway inFrontiers in bioengineering and biotechnology · 2022Article
- One stone two birds: Biosynthesis of 3-hydroxypropionic acid from COSynthetic and systems biotechnology · 2021Article
- Biosynthesis pathways and strategies for improving 3-hydroxypropionic acid production in bacteria.World journal of microbiology & biotechnology · 2021Review
- Hyperproduction of 3-hydroxypropionate by Halomonas bluephagenesis.Nature communications · 2021Article
- Intensifying niacin-based biosynthesis of NADBiotechnology letters · 2021Article
- Improved Production of Pyrroloquinoline Quinone by Simultaneous Augmentation of Its Synthesis Gene Expression and Glucose Metabolism in Klebsiella pneumoniae.Current microbiology · 2020Article
- Resolving the formidable barrier of oxygen transferring rate (OTR) in ultrahigh-titer bioconversion/biocatalysis by a sealed-oxygen supply biotechnology (SOS).Biotechnology for biofuels · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
3-Hydroxypropionic acid (3-HP) is an important platform chemical proposed by the United States Department of Energy. 3-HP can be converted to a series of bulk chemicals. Biological production of 3-HP has made great progress in recent years. However, low yield of 3-HP restricts its commercialization. In this study, systematic optimization was conducted towards high-yield production of 3-HP in Klebsiella pneumoniae. We first investigated appropriate promoters for the key enzyme (aldehyde dehydrogenase, ALDH) in 3-HP biosynthesis, and found that IPTG-inducible tac promoter enabled overexpression of an endogenous ALDH (PuuC) in K. pneumoniae. We optimized the metabolic flux and found that blocking the synthesis of lactic acid and acetic acid significantly increased the production of 3-HP. Additionally, fermentation conditions were optimized and scaled-up cultivation were investigated. The highest 3-HP titer was observed at 83.8 g/L with a high conversion ratio of 54% on substrate glycerol. Furthermore, a flux distribution model of glycerol metabolism in K. pneumoniae was proposed based on in silico analysis. To our knowledge, this is the highest 3-HP production in K. pneumoniae. This work has significantly advanced biological production of 3-HP from renewable carbon sources.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.