ArticleScientific reports2016
Interconnection of post-transcriptional regulation: The RNA-binding protein Hfq is a novel target of the Lon protease in Pseudomonas aeruginosa.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- Function and transcription of the lon gene in Xanthomonas campestris pv. campestris.Antonie van Leeuwenhoek · 2025Article
- Direct and indirect pathways linking the Lon protease to motility behaviors in the pathogen Pseudomonas aeruginosa.PLoS pathogens · 2025Article
- Evolution of PqsE as amBio · 2025Article
- Translational regulation by Hfq-Crc assemblies emerges from polymorphic ribonucleoprotein folding.The EMBO journal · 2023Article
- Scanning mutagenesis of RNA-binding protein ProQ reveals a quality control role for the Lon protease.RNA (New York, N.Y.) · 2021Article
- Wisdom of the crowds: A suggested polygenic plan for small-RNA-mediated regulation in bacteria.iScience · 2021Article
- Specific and Global RNA Regulators inInternational journal of molecular sciences · 2021Review
- The global regulator Hfq exhibits far more extensive and intensive regulation than Crc in Pseudomonas protegens H78.Molecular plant pathology · 2021Article
- Article
- Lon Protease Is Important for Growth Under Stressful Conditions and Pathogenicity of the Phytopathogen, BacteriumInternational journal of molecular sciences · 2020Article
- Iron Limitation inFrontiers in microbiology · 2020Article
- Enhancement of immune response against Bordetella spp. by disrupting immunomodulation.Scientific reports · 2019Article
- Article
- Interplay between the catabolite repression control protein Crc, Hfq and RNA in Hfq-dependent translational regulation in Pseudomonas aeruginosa.Nucleic acids research · 2018Article
- Quick change: post-transcriptional regulation in Pseudomonas.FEMS microbiology letters · 2017Review
- A novel small RNA is important for biofilm formation and pathogenicity in Pseudomonas aeruginosa.PloS one · 2017Article
- Cross-regulation by CrcZ RNA controls anoxic biofilm formation in Pseudomonas aeruginosa.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Besides being a major opportunistic human pathogen, Pseudomonas aeruginosa can be found in a wide range of environments. This versatility is linked to complex regulation, which is achieved through the action of transcriptional regulators, and post-transcriptional regulation by intracellular proteases including Lon. Indeed, lon mutants in this species show defects in motility, biofilm formation, pathogenicity and fluoroquinolone resistance. Here, the proteomic approach stable isotope labeling by amino acids in cell culture (SILAC) was used to search for novel proteolytic targets. One of the proteins that accumulated in the lon mutant was the RNA-binding protein Hfq. Further experiments demonstrated the ability of Lon to degrade Hfq in vitro. Also, overexpression of the hfq gene in the wild-type strain led to partial inhibition of swarming, swimming and twitching motilities, indicating that Hfq accumulation could contribute to the phenotypes displayed by Lon mutants. Hfq overexpression also led to the upregulation of the small regulatory RNA PhrS. Analysis of the phenotypes of strains lacking or overexpressing this sRNA indicated that the Lon protease might be indirectly regulating the levels and activity of sRNAs via Hfq. Overall, this study revealed new links in the complex regulatory chain that controls multicellular behaviours in P. aeruginosa.
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